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Methods in Molecular Biology

Schwann Cells

Methods and Protocols

Editors: Monje, Paula V., Kim, Haesun A. (Eds.)

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  • Include cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7649-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
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  • Immediate eBook download after purchase
Hardcover $139.00
price for USA in USD
  • ISBN 978-1-4939-7648-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week).
  • Due: March 30, 2019
  • ISBN 978-1-4939-9254-6
  • Free shipping for individuals worldwide
About this book

This volume presents an assortment of traditional and emerging experimental procedures relevant to Schwann cell research. The chapters are divided into four parts. Part I contains protocols for in vitro culture, purification, and characterization of primary Schwann cells from diverse species and stages of nerve development. It also contains protocols to create cancer cell lines and engineered Schwann cells from unconventional sources via chemical conversion, induced differentiation or genetic intervention. Parts II and III outline a wide range of methodologies used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of peripheral nerve development, cancer, axon degeneration/regeneration, and myelination. Last but not least, part IV outlines protocols for Schwann cell production, collection, labeling and transplantation in the injured peripheral nerve and spinal cord of experimental animals and human subjects.

Authoritative and practical, Schwann Cells: Methods and Protocols aims to aid both experienced and new investigators to make progress in their research endeavors involving Schwann cells.

Table of contents (31 chapters)

Table of contents (31 chapters)
  • Isolation of Schwann Cell Precursors from Rodents

    Pages 3-15

    Mirsky, Rhona (et al.)

  • Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies

    Pages 17-37

    Maurel, Patrice

  • Isolation and Expansion of Schwann Cells from Transgenic Mouse Models

    Pages 39-48

    Kim, Jihyun (et al.)

  • Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers

    Pages 49-66

    Andersen, Natalia D. (et al.)

  • Detailed Protocols for the Isolation, Culture, Enrichment and Immunostaining of Primary Human Schwann Cells

    Pages 67-86

    Weiss, Tamara (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7649-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA in USD
  • ISBN 978-1-4939-7648-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week).
  • Due: March 30, 2019
  • ISBN 978-1-4939-9254-6
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Schwann Cells
Book Subtitle
Methods and Protocols
Editors
  • Paula V. Monje
  • Haesun A. Kim
Series Title
Methods in Molecular Biology
Series Volume
1739
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7649-2
DOI
10.1007/978-1-4939-7649-2
Hardcover ISBN
978-1-4939-7648-5
Softcover ISBN
978-1-4939-9254-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 488
Number of Illustrations
27 b/w illustrations, 68 illustrations in colour
Topics