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Antibiotic Resistance Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Stephen H. Gillespie

    1. School of Medicine, University of St. Andrews, North Haugh, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1736)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Methods for Measuring the Production of Quorum Sensing Signal Molecules

    • Manuel Alcalde-Rico, José Luis Martínez
    Pages 1-15

  3. Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics

    • Gareth McVicker, Tomasz K. Prajsnar, Simon J. Foster
    Pages 17-27

  4. Method for Detecting and Studying Genome-Wide Mutations in Single Living Cells in Real Time

    • Marina Elez, Lydia Robert, Ivan Matic
    Pages 29-39

  5. Detecting Phenotypically Resistant Mycobacterium tuberculosis Using Wavelength Modulated Raman Spectroscopy

    • Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, Kishan Dholakia, Stephen H. Gillespie
    Pages 41-50

  6. A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics

    • Charlotte L. Hendon-Dunn, Stephen R. Thomas, Stephen C. Taylor, Joanna Bacon
    Pages 51-57

  7. Application of Continuous Culture for Assessing Antibiotic Activity Against Mycobacterium tuberculosis

    • Charlotte L. Hendon-Dunn, Saba Anwar, Christopher Burton, Joanna Bacon
    Pages 59-73

  8. Real-Time Digital Bright Field Technology for Rapid Antibiotic Susceptibility Testing

    • Chiara Canali, Erik Spillum, Martin Valvik, Niels Agersnap, Tom Olesen
    Pages 75-84

  9. Enhanced Methodologies for Detecting Phenotypic Resistance in Mycobacteria

    • Robert J. H. Hammond, Vincent O. Baron, Sam Lipworth, Stephen H. Gillespie
    Pages 85-94

  10. Methods to Determine Mutational Trajectories After Experimental Evolution of Antibiotic Resistance

    • Douglas L. Huseby, Diarmaid Hughes
    Pages 95-103

  11. Selection of ESBL-Producing E. coli in a Mouse Intestinal Colonization Model

    • Frederik Boëtius Hertz, Karen Leth Nielsen, Niels Frimodt-Møller
    Pages 105-115

  12. Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa

    • Leticia Muraro Wildner, Katherine A. Gould, Simon J. Waddell
    Pages 117-128

  13. Direct in Gel Genomic Detection of Antibiotic Resistance Genes in S1 Pulsed Field Electrophoresis Gels

    • Mark A. Toleman
    Pages 129-136

  14. Using RT qPCR for Quantifying Mycobacteria marinum from In Vitro and In Vivo Samples

    • Han Xaio, Stephen H. Gillespie
    Pages 137-145

  15. Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics

    • Tomasz K. Prajsnar, Gareth McVicker, Alexander Williams, Stephen A. Renshaw, Simon J. Foster
    Pages 147-156

  16. A Method to Evaluate Persistent Mycobacterium tuberculosis In Vitro and in the Cornell Mouse Model of Tuberculosis

    • Yanmin Hu, Anthony Coates
    Pages 157-166

  17. Back Matter

    Pages 167-168
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Keywords

About this book

This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response.  New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • School of Medicine, University of St. Andrews, North Haugh, United Kingdom

    Stephen H. Gillespie

Bibliographic Information

  • Book Title: Antibiotic Resistance Protocols

  • Editors: Stephen H. Gillespie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7638-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7636-2Published: 11 January 2018

  • Softcover ISBN: 978-1-4939-8534-0Published: 09 June 2019

  • eBook ISBN: 978-1-4939-7638-6Published: 10 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: X, 168

  • Number of Illustrations: 27 b/w illustrations, 5 illustrations in colour

  • Topics: Microbiology

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