Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1737)
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Table of contents (21 protocols)
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Front Matter
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Function and Differential Expression of sRNA
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Front Matter
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Quantitation and Subcellular Localisation of sRNA
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Front Matter
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Cofactors of sRNA-Based Regulation
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Front Matter
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About this book
This volume details the most important methods used for studying prokaryotic non-coding RNAs and their protein accomplices. Chapters present methods in sections covering different aspects of the biology of that field: identification of ncRNAs, their differential expression, characterization of their structure, abundance, intracellular location and function, their interaction with RNA binding proteins, and plausible applications of ncRNA elements in the rapidly emerging field of synthetic biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Regulatory RNA: Methods and Protocols serves as a guidebook for scientists working toward the development of new tools and proceduresfor the vital field of sRNA biology.
Editors and Affiliations
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Université Paris Diderot & LLB/CEA/CNRS, Gif-sur-Yvette, France
Véronique Arluison
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Laboratorio de Bioquímica, Microbiología e Interacciones, Biológicas en el Suelo, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes-CONICET, Bernal, Argentina
Claudio Valverde
Bibliographic Information
Book Title: Bacterial Regulatory RNA
Book Subtitle: Methods and Protocols
Editors: Véronique Arluison, Claudio Valverde
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7634-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2018
Hardcover ISBN: 978-1-4939-7633-1Published: 27 February 2018
Softcover ISBN: 978-1-4939-9251-5Published: 10 December 2019
eBook ISBN: 978-1-4939-7634-8Published: 26 February 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 395
Number of Illustrations: 22 b/w illustrations, 62 illustrations in colour
Topics: Human Genetics