Skip to main content
  • Book
  • © 2018

MicroRNA Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1733)

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 139.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
  2. The MicroRNA

    • Shao-Yao Ying, Donald C. Chang, Shi-Lung Lin
    Pages 1-25
  3. Target mRNA-Driven Biogenesis of Cognate MicroRNAs In Vitro

    • Mainak Bose, Suvendra N. Bhattacharyya
    Pages 27-39
  4. Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid

    • Juliano Da Silveira, Gabriella M. Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A. Winger, Gerrit J. Bouma
    Pages 53-63
  5. Profiling of MicroRNAs in the Biofluids of Livestock Species

    • Jason Ioannidis, Judith Risse, F. Xavier Donadeu
    Pages 65-77
  6. Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation

    • Max Hübner, Pedro A. F. Galante, Simone Kreth, Ludwig Christian Hinske
    Pages 87-92
  7. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs

    • Shi-Lung Lin, Shao-Yao Ying
    Pages 107-126
  8. Isolation and Identification of Gene-Specific MicroRNAs

    • Shi-Lung Lin, Donald C. Chang, Shao-Yao Ying
    Pages 173-180
  9. Gene Manipulation with Micro RNAs at Single-Human Cancer Cell

    • Andres Stucky, Xuelian Chen, Jiang F. Zhong
    Pages 215-223
  10. Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis

    • Alyne Simões, Zujian Chen, Yan Zhao, Lin Chen, Virgilia Macias, Luisa A. DiPietro et al.
    Pages 225-237

About this book

This volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that’s cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource thatprovides researchers with a comprehensive study of miRNAs and its numerous components and uses.



Editors and Affiliations

  • Department of Integrative Anatomical Sciences, USC, Keck School of Medicine, Los Angeles, USA

    Shao-Yao Ying

Bibliographic Information

  • Book Title: MicroRNA Protocols

  • Editors: Shao-Yao Ying

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7601-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7600-3Published: 13 February 2018

  • Softcover ISBN: 978-1-4939-9250-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7601-0Published: 12 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 319

  • Number of Illustrations: 9 b/w illustrations, 41 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 139.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access