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Methods in Molecular Biology

AMPK

Methods and Protocols

Editors: Neumann, Dietbert, Viollet, Benoit (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7598-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-4939-7597-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week). Pre-ordered printed titles are excluded from promotions.
  • Due: March 12, 2019
  • ISBN 978-1-4939-9249-2
  • Free shipping for individuals worldwide
About this book

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.


Table of contents (37 chapters)

Table of contents (37 chapters)
  • Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (α1β1γ1)

    Pages 1-14

    Hubbard, Julia A. (et al.)

  • Visualizing AMPK Drug Binding Sites Through Crystallization of Full-Length Phosphorylated α2β1γ1 Heterotrimer

    Pages 15-27

    Langendorf, Christopher G. (et al.)

  • Biophysical Interactions of Direct AMPK Activators

    Pages 29-55

    Kurumbail, Ravi G. (et al.)

  • Biochemical Measurement of Glycogen: Method to Investigate the AMPK-Glycogen Relationship

    Pages 57-67

    Possik, Elite (et al.)

  • Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK

    Pages 69-86

    Fyffe, Fiona A. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7598-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-1-4939-7597-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • Customers within the U.S. and Canada please contact Customer Service at +1-800-777-4643, Latin America please contact us at +1-212-460-1500 (24 hours a day, 7 days a week). Pre-ordered printed titles are excluded from promotions.
  • Due: March 12, 2019
  • ISBN 978-1-4939-9249-2
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
AMPK
Book Subtitle
Methods and Protocols
Editors
  • Dietbert Neumann
  • Benoit Viollet
Series Title
Methods in Molecular Biology
Series Volume
1732
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7598-3
DOI
10.1007/978-1-4939-7598-3
Hardcover ISBN
978-1-4939-7597-6
Softcover ISBN
978-1-4939-9249-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 626
Number of Illustrations
42 b/w illustrations, 72 illustrations in colour
Topics