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  • © 2018

Clinical Metabolomics

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols for the use of clinical metabolomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1730)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
  2. Clinical Metabolomics and Lipidomics

    1. Front Matter

      Pages 1-1
    2. Metabolomics as a Tool to Understand Pathophysiological Processes

      • Julijana Ivanisevic, Aurelien Thomas
      Pages 3-28
    3. Metabolomics in Immunology Research

      • Bart Everts
      Pages 29-42
  3. LC-MS-Based Metabolomics

    1. Front Matter

      Pages 43-43
    2. LC-MS-Based Metabolomics of Biofluids Using All-Ion Fragmentation (AIF) Acquisition

      • Romanas Chaleckis, Shama Naz, Isabel Meister, Craig E. Wheelock
      Pages 45-58
    3. Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis

      • Jesmond Dalli, Romain A. Colas, Mary E. Walker, Charles N. Serhan
      Pages 59-72
    4. UHPSFC/ESI-MS Analysis of Lipids

      • Miroslav Lísa, Michal Holčapek
      Pages 73-82
    5. Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics

      • Lennart J. van Winden, Olaf van Tellingen, Huub H. van Rossum
      Pages 93-102
    6. LC-MS/MS Analysis of Bile Acids

      • Sabrina Krautbauer, Gerhard Liebisch
      Pages 103-110
    7. LC-MS/MS Analysis of Triglycerides in Blood-Derived Samples

      • Madlen Reinicke, Susen Becker, Uta Ceglarek
      Pages 111-121
    8. LC-MS/MS Analysis of the Epoxides and Diols Derived from the Endocannabinoid Arachidonoyl Ethanolamide

      • Amy A. Rand, Patrick O. Helmer, Bora Inceoglu, Bruce D. Hammock, Christophe Morisseau
      Pages 123-133
    9. Sphingolipid Analysis in Clinical Research

      • Bo Burla, Sneha Muralidharan, Markus R. Wenk, Federico Torta
      Pages 135-162
    10. Shotgun Lipidomics Approach for Clinical Samples

      • Lars F. Eggers, Dominik Schwudke
      Pages 163-174
  4. GC-MS-Based Metabolomics

    1. Front Matter

      Pages 237-237
    2. Exploratory GC/MS-Based Metabolomics of Body Fluids

      • Carole Migné, Stéphanie Durand, Estelle Pujos-Guillot
      Pages 239-246

About this book

This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion.

Editors and Affiliations

  • Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands

    Martin Giera

Bibliographic Information

  • Book Title: Clinical Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Martin Giera

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7592-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7591-4Published: 24 January 2018

  • Softcover ISBN: 978-1-4939-8530-2Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7592-1Published: 23 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 389

  • Number of Illustrations: 17 b/w illustrations, 22 illustrations in colour

  • Topics: Biochemistry, general

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 129.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access