- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume covers some of the most widely used protocols on nanocanonical amino acids, providing details and advice for users to get each method up and running for their chosen application. Chapters have been divided into three parts describing methods for protein production in the test tube, in prokaryotes, and in eukaryotes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Noncanonical Amino Acids: Methods and Protocols aims to provide readers with techniques that enable them to design new experiments and create new areas of research.
- Table of contents (24 chapters)
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Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates
Pages 3-16
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Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis
Pages 17-47
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Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains
Pages 49-65
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Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids
Pages 67-93
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Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids
Pages 97-111
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Table of contents (24 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Noncanonical Amino Acids
- Book Subtitle
- Methods and Protocols
- Editors
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- Edward A. Lemke
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1728
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-4939-7574-7
- DOI
- 10.1007/978-1-4939-7574-7
- Hardcover ISBN
- 978-1-4939-7573-0
- Softcover ISBN
- 978-1-4939-8525-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 411
- Number of Illustrations
- 37 b/w illustrations, 39 illustrations in colour
- Topics
