Methods in Molecular Biology

Noncanonical Amino Acids

Methods and Protocols

Editors: Lemke, Edward A. (Ed.)

  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7574-7
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA in USD
  • ISBN 978-1-4939-7573-0
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About this book

This volume covers some of the most widely used protocols on nanocanonical amino acids, providing details and advice for users to get each method up and running for their chosen application.  Chapters have been divided into three parts describing methods for protein production in the test tube, in prokaryotes, and in eukaryotes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Noncanonical Amino Acids: Methods and Protocols aims to provide readers with techniques that enable them to design new experiments and create new areas of research.

Table of contents (24 chapters)

  • Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates

    Barfield, Robyn M. (et al.)

    Pages 3-16

  • Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis

    Iwane, Yoshihiko (et al.)

    Pages 17-47

  • Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains

    Seki, Eiko (et al.)

    Pages 49-65

  • Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids

    Helma, Jonas (et al.)

    Pages 67-93

  • Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids

    Schmidt, Moritz J. (et al.)

    Pages 97-111

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7574-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA in USD
  • ISBN 978-1-4939-7573-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Noncanonical Amino Acids
Book Subtitle
Methods and Protocols
Editors
  • Edward A. Lemke
Series Title
Methods in Molecular Biology
Series Volume
1728
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7574-7
DOI
10.1007/978-1-4939-7574-7
Hardcover ISBN
978-1-4939-7573-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 411
Number of Illustrations and Tables
37 b/w illustrations, 39 illustrations in colour
Topics