Methods in Molecular Biology

The Retinoblastoma Protein

Editors: Santiago Cardona, Pedro (Ed.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7565-5
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About this book

This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, The Retinoblastoma Protein aims to serve as a guide to assist molecular cancer biologists in their search for understanding of the molecular functions of this preeminent tumor suppressor.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia

    Pages 1-6

    Liehr, Thomas

  • Detection of RB1 Gene Copy Number Variations Using a Multiplex Ligation-Dependent Probe Amplification Method

    Pages 7-18

    Dalay, Nejat

  • A Fluorescent Quantitative Multiplex PCR Method to Detect Copy Number Changes in the RB1 Gene

    Pages 19-28

    Kannabiran, Chitra

  • Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation

    Pages 29-34

    McCormick, Thaís M. (et al.)

  • Detection of Aberrant DNA Methylation Patterns in the RB1 Gene

    Pages 35-47

    Anwar, Sumadi Lukman (et al.)

Buy this book

eBook $84.99
price for USA in USD
  • ISBN 978-1-4939-7565-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $149.99
price for USA in USD
Softcover $109.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
The Retinoblastoma Protein
Editors
  • Pedro Santiago Cardona
Series Title
Methods in Molecular Biology
Series Volume
1726
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7565-5
DOI
10.1007/978-1-4939-7565-5
Hardcover ISBN
978-1-4939-7564-8
Softcover ISBN
978-1-4939-8522-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 200
Number of Illustrations
6 b/w illustrations, 32 illustrations in colour
Topics