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  • © 2018

Circular RNAs

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1724)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
  2. Deep Computational Circular RNA Analytics from RNA-seq Data

    • Tobias Jakobi, Christoph Dieterich
    Pages 9-25
  3. Genome-Wide circRNA Profiling from RNA-seq Data

    • Daphne A. Cooper, Mariela Cortés-López, Pedro Miura
    Pages 27-41
  4. Analysis of Circular RNAs Using the Web Tool CircInteractome

    • Amaresh C. Panda, Dawood B. Dudekula, Kotb Abdelmohsen, Myriam Gorospe
    Pages 43-56
  5. Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization

    • Anne Zirkel, Argyris Papantonis
    Pages 69-75
  6. Single-Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as

    • Christine Kocks, Anastasiya Boltengagen, Monika Piwecka, Agnieszka Rybak-Wolf, Nikolaus Rajewsky
    Pages 77-96
  7. A Highly Efficient Strategy for Overexpressing circRNAs

    • Dawei Liu, Vanessa Conn, Gregory J. Goodall, Simon J. Conn
    Pages 97-105
  8. Northern Blot Analysis of Circular RNAs

    • Tim Schneider, Silke Schreiner, Christian Preußer, Albrecht Bindereif, Oliver Rossbach
    Pages 119-133
  9. Nonradioactive Northern Blot of circRNAs

    • Xiaolin Wang, Ge Shan
    Pages 135-141
  10. Characterization of Circular RNA Concatemers

    • Thomas B. Hansen
    Pages 143-157
  11. Synthesis and Engineering of Circular RNAs

    • Sonja Petkovic, Sabine Müller
    Pages 167-180
  12. Preparation of Circular RNA In Vitro

    • Naoko Abe, Ayumi Kodama, Hiroshi Abe
    Pages 181-192
  13. Discovering circRNA-microRNA Interactions from CLIP-Seq Data

    • Xiao-Qin Zhang, Jian-Hua Yang
    Pages 193-207
  14. Back Matter

    Pages 219-220

About this book

This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.  Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field. 

Editors and Affiliations

  • Department of Internal Medicine III Klaus Tschira Institute for Integrative Computational Cardiology, University Hospital Heidelberg, Heidelberg, Germany

    Christoph Dieterich

  • Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany

    Argyris Papantonis

Bibliographic Information

  • Book Title: Circular RNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Christoph Dieterich, Argyris Papantonis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7562-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7561-7Published: 10 January 2018

  • Softcover ISBN: 978-1-4939-8521-0Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7562-4Published: 08 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 220

  • Number of Illustrations: 17 b/w illustrations, 33 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 69.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 89.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 119.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access