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Methods in Molecular Biology

The Surfaceome

Methods and Protocols

Editors: Boheler, Kenneth R., Gundry, Rebekah L. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $119.00
price for USA in USD
  • ISBN 978-1-4939-7553-2
  • Digitally watermarked, DRM-free
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $159.00
price for USA in USD
About this book

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens

    Pages 3-20

    Williamson, Yulanda M. (et al.)

  • “Shaving” Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins

    Pages 21-29

    Rodríguez-Ortega, Manuel J.

  • Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes

    Pages 31-56

    Kilarski, Witold W. (et al.)

  • Mass Spectrometry-Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and Their Derivatives

    Pages 57-78

    Fujinaka, Chelsea M. (et al.)

  • Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and Its Proteome from Cells in Suspension

    Pages 79-90

    Rose, Rebecca L. (et al.)

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-1-4939-7553-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $159.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
The Surfaceome
Book Subtitle
Methods and Protocols
Editors
  • Kenneth R. Boheler
  • Rebekah L. Gundry
Series Title
Methods in Molecular Biology
Series Volume
1722
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7553-2
DOI
10.1007/978-1-4939-7553-2
Hardcover ISBN
978-1-4939-7551-8
Softcover ISBN
978-1-4939-8518-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 348
Number of Illustrations
21 b/w illustrations, 47 illustrations in colour
Topics