The Surfaceome
Methods and Protocols
Editors: Boheler, Kenneth R., Gundry, Rebekah L. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.
- Table of contents (19 chapters)
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Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens
Pages 3-20
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“Shaving” Live Bacterial Cells with Proteases for Proteomic Analysis of Surface Proteins
Pages 21-29
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Methods for Mapping the Extracellular and Membrane Proteome in the Avian Embryo, and Identification of Putative Vascular Targets or Endothelial Genes
Pages 31-56
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Mass Spectrometry-Based Identification of Extracellular Domains of Cell Surface N-Glycoproteins: Defining the Accessible Surfaceome for Immunophenotyping Stem Cells and Their Derivatives
Pages 57-78
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Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and Its Proteome from Cells in Suspension
Pages 79-90
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Table of contents (19 chapters)
- Download Preface 1 PDF (29 KB)
- Download Sample pages 1 PDF (129.1 KB)
- Download Table of contents PDF (296.8 KB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- The Surfaceome
- Book Subtitle
- Methods and Protocols
- Editors
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- Kenneth R. Boheler
- Rebekah L. Gundry
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1722
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-4939-7553-2
- DOI
- 10.1007/978-1-4939-7553-2
- Hardcover ISBN
- 978-1-4939-7551-8
- Softcover ISBN
- 978-1-4939-8518-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 348
- Number of Illustrations
- 21 b/w illustrations, 47 illustrations in colour
- Topics
