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Methods in Molecular Biology

Innate Immune Activation

Methods and Protocols

Editors: De Nardo, Dominic, De Nardo, Christine (Eds.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7519-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-7518-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8508-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Innate Immune Activation: Methods and Protocols aims to be a useful and informative reference tool for further study into this vital field.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Emerging Concepts in Innate Immunity

    Pages 1-18

    Pelka, Karin (et al.)

  • Bioinformatic Assessment of Macrophage Activation by the Innate Immune System

    Pages 19-40

    Ulas, Thomas (et al.)

  • Generation of Genetic Knockouts in Myeloid Cell Lines Using a Lentiviral CRISPR/Cas9 System

    Pages 41-55

    Baker, Paul J. (et al.)

  • Modeling Primary Human Monocytes with the Trans–Differentiation Cell Line BLaER1

    Pages 57-66

    Gaidt, Moritz M. (et al.)

  • Measurement of NF-κB Activation in TLR-Activated Macrophages

    Pages 67-78

    Ernst, Orna (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7519-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-7518-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8508-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Innate Immune Activation
Book Subtitle
Methods and Protocols
Editors
  • Dominic De Nardo
  • Christine De Nardo
Series Title
Methods in Molecular Biology
Series Volume
1714
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7519-8
DOI
10.1007/978-1-4939-7519-8
Hardcover ISBN
978-1-4939-7518-1
Softcover ISBN
978-1-4939-8508-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 251
Number of Illustrations
14 b/w illustrations, 40 illustrations in colour
Topics