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Methods in Molecular Biology

Next Generation Sequencing

Methods and Protocols

Editors: Head, Steven R., Ordoukhanian, Phillip, Salomon, Daniel R. (Eds.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7514-3
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  • Included format: EPUB, PDF
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Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-7512-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8507-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications,  method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field. 

Table of contents (16 chapters)

Table of contents (16 chapters)
  • An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome

    Pages 1-18

    Jin, Hyun Yong (et al.)

  • Measuring Nascent Transcripts by Nascent-seq

    Pages 19-26

    Chen, Fei Xavier (et al.)

  • Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis

    Pages 27-42

    Deleye, Lieselot (et al.)

  • Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure

    Pages 43-51

    Karamitros, Timokratis, Ph.D. (et al.)

  • Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing

    Pages 53-70

    Pope, Bernard J. (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7514-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-7512-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8507-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Next Generation Sequencing
Book Subtitle
Methods and Protocols
Editors
  • Steven R. Head
  • Phillip Ordoukhanian
  • Daniel R. Salomon
Series Title
Methods in Molecular Biology
Series Volume
1712
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7514-3
DOI
10.1007/978-1-4939-7514-3
Hardcover ISBN
978-1-4939-7512-9
Softcover ISBN
978-1-4939-8507-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 266
Number of Illustrations
10 b/w illustrations, 41 illustrations in colour
Topics