Next Generation Sequencing
Methods and Protocols
Editors: Head, Steven R., Ordoukhanian, Phillip, Salomon, Daniel R. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications, method called “Hi-Plex” to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field.
- Table of contents (16 chapters)
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An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome
Pages 1-18
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Measuring Nascent Transcripts by Nascent-seq
Pages 19-26
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Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
Pages 27-42
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Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure
Pages 43-51
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Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing
Pages 53-70
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Next Generation Sequencing
- Book Subtitle
- Methods and Protocols
- Editors
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- Steven R. Head
- Phillip Ordoukhanian
- Daniel R. Salomon
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1712
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-4939-7514-3
- DOI
- 10.1007/978-1-4939-7514-3
- Hardcover ISBN
- 978-1-4939-7512-9
- Softcover ISBN
- 978-1-4939-8507-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 266
- Number of Illustrations
- 10 b/w illustrations, 41 illustrations in colour
- Topics
