- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
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This third edition volume expands on the previous editions by providing a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.
- Table of contents (35 chapters)
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A Summary of the Biological Processes, Disease-Associated Changes, and Clinical Applications of DNA Methylation
Pages 3-30
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Considerations for Design and Analysis of DNA Methylation Studies
Pages 31-46
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Quantification of Global DNA Methylation Levels by Mass Spectrometry
Pages 49-58
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Antibody-Based Detection of Global Nuclear DNA Methylation in Cells, Tissue Sections, and Mammalian Embryos
Pages 59-80
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Whole-Genome Bisulfite Sequencing Using the Ovation® Ultralow Methyl-Seq Protocol
Pages 83-104
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Table of contents (35 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- DNA Methylation Protocols
- Editors
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- Jorg Tost
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1708
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- eBook ISBN
- 978-1-4939-7481-8
- DOI
- 10.1007/978-1-4939-7481-8
- Hardcover ISBN
- 978-1-4939-7479-5
- Softcover ISBN
- 978-1-4939-8499-2
- Series ISSN
- 1064-3745
- Edition Number
- 3
- Number of Pages
- XVIII, 704
- Number of Illustrations
- 35 b/w illustrations, 67 illustrations in colour
- Topics
*immediately available upon purchase as print book shipments may be delayed due to the COVID-19 crisis. ebook access is temporary and does not include ownership of the ebook. Only valid for books with an ebook version. Springer Reference Works and instructor copies are not included.
