Methods in Molecular Biology

Chaperones

Methods and Protocols

Editors: Calderwood, Stuart K., Prince, Thomas L. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-7477-1
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Hardcover $219.99
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Softcover $149.99
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About this book

This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response.  Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents (29 chapters)

Table of contents (29 chapters)
  • Targeted Deletion of Hsf1, 2, and 4 Genes in Mice

    Pages 1-22

    Jin, Xiongjie (et al.)

  • Role of Heat Shock Factors in Stress-Induced Transcription

    Pages 23-34

    Murshid, Ayesha (et al.)

  • Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter

    Pages 35-45

    Kijima, Toshiki (et al.)

  • Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay

    Pages 47-58

    Taipale, Mikko

  • Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay

    Pages 59-74

    Waarde-Verhagen, Maria A. W. H. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-7477-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
  • Institutional customers should get in touch with their account manager
Hardcover $219.99
price for USA in USD
Softcover $149.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Chaperones
Book Subtitle
Methods and Protocols
Editors
  • Stuart K. Calderwood
  • Thomas L. Prince
Series Title
Methods in Molecular Biology
Series Volume
1709
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7477-1
DOI
10.1007/978-1-4939-7477-1
Hardcover ISBN
978-1-4939-7476-4
Softcover ISBN
978-1-4939-8498-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIX, 445
Number of Illustrations
33 b/w illustrations, 36 illustrations in colour
Topics