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Methods in Molecular Biology

DNA Topoisomerases

Methods and Protocols

Editors: Drolet, Marc (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7459-7
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Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-7458-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8492-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume looks at the evolution of the topo field over the last year and discusses how DNA topoisomerases are directly and indirectly involved in numerous cellular processes. The chapters in this book cover various topics, such as type 1 topos with an evolutionary perspective; antimicrobial agents targeting topos; experimental protocols related to DNA topology, including a basic technique to measure DNA supercoiling in bacteria; protocols for in vitro studies, including assays to detect RNA topo activity; topo inhibitors; and a study of plasmid-mediated quinolone resistance in bacteria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, DNA Topoisomerases: Methods and Protoc

ols is intended for specialists and non-specialists with an overview of selected hot topics in the field, and the latest protocols to study DNA topology, topoisomerases functions and inhibition by various compounds.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Type IA DNA Topoisomerases: A Universal Core and Multiple Activities

    Garnier, Florence (et al.)

    Pages 1-20

  • Topoisomerase I and Genome Stability: The Good and the Bad

    Cho, Jang-Eun (et al.)

    Pages 21-45

  • DNA Topoisomerases as Targets for Antibacterial Agents

    Hiasa, Hiroshi

    Pages 47-62

  • DNA Supercoiling Measurement in Bacteria

    Liu, Yingting (et al.)

    Pages 63-73

  • DNA Catenation Reveals the Dynamics of DNA Topology During Replication

    Castán, Alicia (et al.)

    Pages 75-86

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7459-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-7458-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8492-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
DNA Topoisomerases
Book Subtitle
Methods and Protocols
Editors
  • Marc Drolet
Series Title
Methods in Molecular Biology
Series Volume
1703
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7459-7
DOI
10.1007/978-1-4939-7459-7
Hardcover ISBN
978-1-4939-7458-0
Softcover ISBN
978-1-4939-8492-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 328
Number of Illustrations
46 b/w illustrations, 21 illustrations in colour
Topics