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Methods in Molecular Biology

Bacterial Multidrug Exporters

Methods and Protocols

Editors: Yamaguchi, Akihito, Nishino, Kunihiko (Eds.)

  • Includes cutting-edge methods and protocols for the study of bacterial multidrug exporters
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7454-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-7452-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria. Beginning with protocols for the structural analysis of bacterial multidrug exporters, the book continues with sections on biochemical and bioengineering analysis, computational analysis, biomedical approaches, as well as advanced technologies expected to be applied to multidrug efflux transport studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bacterial Multidrug Exporters: Methods and Protocols serves as an ideal guide to

this fast moving field of study.

Table of contents (19 chapters)

  • High-Resolution Crystallographic Analysis of AcrB Using Designed Ankyrin Repeat Proteins (DARPins)

    Tam, Heng Keat (et al.)

    Pages 3-24

  • Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter AcrB in Physiologically Relevant Asymmetric Crystals

    Nakashima, Ryosuke (et al.)

    Pages 25-36

  • Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitors

    Kusakizako, Tsukasa (et al.)

    Pages 37-57

  • Crystallographic Analysis of the CusBA Heavy-Metal Efflux Complex of Escherichia coli

    Delmar, Jared A. (et al.)

    Pages 59-70

  • Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis

    Du, Dijun (et al.)

    Pages 71-81

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7454-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-7452-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Multidrug Exporters
Book Subtitle
Methods and Protocols
Editors
  • Akihito Yamaguchi
  • Kunihiko Nishino
Series Title
Methods in Molecular Biology
Series Volume
1700
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7454-2
DOI
10.1007/978-1-4939-7454-2
Hardcover ISBN
978-1-4939-7452-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 355
Number of Illustrations
38 b/w illustrations, 53 illustrations in colour
Topics