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Methods in Molecular Biology

Erythropoiesis

Methods and Protocols

Editors: Lloyd, Joyce A. (Ed.)

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  • Includes cutting-edge methods and protocols for the study of¬†erythropoiesisProvides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7428-3
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Softcover $169.99
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About this book

This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Erythropoiesis: Methods and Protocols provides a definitive source for molecular and cellular biology protocols to study erythropoiesis and to move this vital field forward.

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Table of contents (18 chapters)

Table of contents (18 chapters)
  • An Introduction to Erythropoiesis Approaches

    Pages 1-10

    Lloyd, Joyce A.

  • Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis

    Pages 11-36

    Kafina, Martin D. (et al.)

  • Mouse Models of Erythropoiesis and Associated Diseases

    Pages 37-65

    Parker, Matthew P. (et al.)

  • Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells

    Pages 67-89

    McIver, Skye C. (et al.)

  • Stress Erythropoiesis Model Systems

    Pages 91-102

    Bennett, Laura F. (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-7428-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-7427-6
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8483-1
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Erythropoiesis
Book Subtitle
Methods and Protocols
Editors
  • Joyce A. Lloyd
Series Title
Methods in Molecular Biology
Series Volume
1698
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7428-3
DOI
10.1007/978-1-4939-7428-3
Hardcover ISBN
978-1-4939-7427-6
Softcover ISBN
978-1-4939-8483-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 298
Number of Illustrations
7 b/w illustrations, 55 illustrations in colour
Topics

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