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Polyamines

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Rubén Alcázar

    1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

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  2. Antonio F. Tiburcio

    1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1694)

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About this book

This volume provides readers with a collection of the latest protocols used by researchers to study polyamines (PA). The chapters in this book cover various topics, such as quantification of different polyamines and conjugates, subcellular localization studies, transport, DNA methylation, ODC regulation, genetic and phenotyping analyses, genome-wide association mapping, polyamine applications and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Polyamines: Methods and Protocols is a useful reference for researchers looking to advance and stimulate their knowledge of polyamines.


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Table of contents (41 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Polyamines in the Context of Metabolic Networks

    • Wegi Wuddineh, Rakesh Minocha, Subhash C. Minocha
    Pages 1-23

  3. Molecules for Sensing Polyamines and Transducing Their Action in Plants

    • Tomonobu Kusano, G. H. M. Sagor, Thomas Berberich
    Pages 25-35

  4. Polyamine Metabolism Responses to Biotic and Abiotic Stress

    • Fernando M. Romero, Santiago J. Maiale, Franco R. Rossi, Maria Marina, Oscar A. Ruíz, Andrés Gárriz
    Pages 37-49

  5. Thermospermine: An Evolutionarily Ancient but Functionally New Compound in Plants

    • Taku Takahashi
    Pages 51-59

  6. Determination of Polyamines by Capillary Electrophoresis Using Salicylaldehyde-5-Sulfonate as a Derivatizing Reagent

    • Takashi Kaneta
    Pages 61-68

  7. Detection of Thermospermine and Spermine by HPLC in Plants

    • Taku Takahashi, Ayaka Takano, Jun-Ichi Kakehi
    Pages 69-73

  8. Development of Amine-Oxidase-Based Biosensors for Spermine and Spermidine Analysis

    • Cristina Tortolini, Gabriele Favero, Franco Mazzei
    Pages 75-80

  9. Identification of Branched-Chain Polyamines in Hyperthermophiles

    • Ryota Hidese, Wakao Fukuda, Masaru Niitsu, Shinsuke Fujiwara
    Pages 81-94

  10. Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry

    • Laura Torras-Claveria, Jaume Bastida, Francesc Viladomat, Antonio F. Tiburcio
    Pages 95-104

  11. Analysis of Glutathione in Biological Samples by HPLC Involving Pre-Column Derivatization with o-Phthalaldehyde

    • Yongqing Hou, Xilong Li, Zhaolai Dai, Zhenlong Wu, Fuller W. Bazer, Guoyao Wu
    Pages 105-115

  12. Determination of Arginine and Ornithine Decarboxylase Activities in Plants

    • Rubén Alcázar, Antonio F. Tiburcio
    Pages 117-122

  13. Determination of S-Adenosylmethionine Decarboxylase Activity in Plants

    • Antonio F. Tiburcio, Rubén Alcázar
    Pages 123-128

  14. Determination of Copper Amine Oxidase Activity in Plant Tissues

    • Riccardo Angelini, Alessandra Cona, Paraskevi Tavladoraki
    Pages 129-139

  15. Determination of di−/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay

    • Panagiotis N. Moschou
    Pages 141-147

  16. Pentamine as a Substrate for Measuring Spermine Oxidase Activity

    • Koichi Takao, Yoshiaki Sugita
    Pages 149-154

  17. Spectrophotometric Quantification of Reactive Oxygen, Nitrogen and Sulfur Species in Plant Samples

    • Chrystalla Antoniou, Andreas Savvides, Egli C. Georgiadou, Vasileios Fotopoulos
    Pages 155-161

  18. Novel Route for Agmatine Catabolism in Aspergillus niger: 4-Guanidinobutyrase Assay

    • Tejaswani Saragadam, Narayan S. Punekar
    Pages 163-172

  19. Determination of Transglutaminase Activity in Plants

    • S. Del Duca, P. L. R. Bonner, I. Aloisi, D. Serafini-Fracassini, G. Cai
    Pages 173-200

  20. Procedures for ADC Immunoblotting and Immunolocalization for Transmission Electron Microscopy During Organogenic Nodule Formation in Hop

    • Ana Margarida Fortes, Jose M. Seguí-Simarro
    Pages 201-214
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Editors and Affiliations

  • Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar, Antonio F. Tiburcio

Bibliographic Information

  • Book Title: Polyamines

  • Book Subtitle: Methods and Protocols

  • Editors: Rubén Alcázar, Antonio F. Tiburcio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7398-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7397-2Published: 28 October 2017

  • Softcover ISBN: 978-1-4939-8473-2Published: 23 May 2018

  • eBook ISBN: 978-1-4939-7398-9Published: 27 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 512

  • Number of Illustrations: 39 b/w illustrations, 55 illustrations in colour

  • Topics: Biochemistry, general

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