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Methods in Molecular Biology

Cellular Quiescence

Methods and Protocols

Editors: Lacorazza, H. Daniel (Ed.)

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  • Includes cutting-edge methods and protocols for the study of cellular quiescence
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7371-2
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-7370-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8465-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches

    Pages 1-25

    So, Wai-Kin (et al.)

  • An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts

    Pages 27-47

    Mitra, Mithun (et al.)

  • Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining

    Pages 49-57

    Eddaoudi, Ayad (et al.)

  • Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells

    Pages 59-67

    Azari, Hassan (et al.)

  • Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System

    Pages 69-78

    Chicheportiche, Alexandra (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7371-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-7370-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8465-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cellular Quiescence
Book Subtitle
Methods and Protocols
Editors
  • H. Daniel Lacorazza
Series Title
Methods in Molecular Biology
Series Volume
1686
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-7371-2
DOI
10.1007/978-1-4939-7371-2
Hardcover ISBN
978-1-4939-7370-5
Softcover ISBN
978-1-4939-8465-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 303
Number of Illustrations
17 b/w illustrations, 43 illustrations in colour
Topics