Skip to main content
SpringerLink
Log in

Protein Engineering

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Uwe T. Bornscheuer

    1. Department of Biotechnology and Enzyme Catalysis Institute of Biochemistry, Greifswald University, Greifswald, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Matthias Höhne

    1. Protein Biochemistry Institute of Biochemistry, Greifswald University, Greifswald, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1685)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 129.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Protein Engineering: Past, Present, and Future

    • Stefan Lutz, Samantha M. Iamurri
    Pages 1-12

  3. Computational Protocols

    1. Front Matter

      Pages 13-13
      Download chapter PDF

    2. Rational and Semirational Protein Design

      • Ivan V. Korendovych
      Pages 15-23

    3. Computational Analysis of Protein Tunnels and Channels

      • Jan Brezovsky, Barbora Kozlikova, Jiri Damborsky
      Pages 25-42

    4. YASARA: A Tool to Obtain Structural Guidance in Biocatalytic Investigations

      • Henrik Land, Maria Svedendahl Humble
      Pages 43-67

    5. A Computational Library Design Protocol for Rapid Improvement of Protein Stability: FRESCO

      • Hein J. Wijma, Maximilian J. L. J. Fürst, Dick B. Janssen
      Pages 69-85

    6. Directed Evolution of Proteins Based on Mutational Scanning

      • Carlos G. Acevedo-Rocha, Matteo Ferla, Manfred T. Reetz
      Pages 87-128

  4. Protein Library Expression

    1. Front Matter

      Pages 129-129
      Download chapter PDF

    2. A Brief Guide to the High-Throughput Expression of Directed Evolution Libraries

      • Ana Luísa Ribeiro, Mario Mencía, Aurelio Hidalgo
      Pages 131-143

    3. Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in E. coli Using MTP Shakers

      • Sandy Schmidt, Mark Dörr, Uwe T. Bornscheuer
      Pages 145-156

    4. Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification

      • Javier Santos-Aberturas, Mark Dörr, Uwe T. Bornscheuer
      Pages 157-170

    5. Functional Analysis of Membrane Proteins Produced by Cell-Free Translation

      • Srujan Kumar Dondapati, Doreen A. Wüstenhagen, Stefan Kubick
      Pages 171-186Open Access

  5. Screening and Selection Assays

    1. Front Matter

      Pages 187-187
      Download chapter PDF

    2. Practical Considerations Regarding the Choice of the Best High-Throughput Assay

      • Carolin Mügge, Robert Kourist
      Pages 189-208

    3. High-Throughput Screening Assays for Lipolytic Enzymes

      • Alexander Fulton, Marc R. Hayes, Ulrich Schwaneberg, Jörg Pietruszka, Karl-Erich Jaeger
      Pages 209-231

    4. Continuous High-Throughput Colorimetric Assays for α-Transaminases

      • Egon Heuson, Jean-Louis Petit, Franck Charmantray, Véronique de Bérardinis, Thierry Gefflaut
      Pages 233-245

    5. Colorimetric High-Throughput Screening Assays for the Directed Evolution of Fungal Laccases

      • Isabel Pardo, Susana Camarero
      Pages 247-254

    6. Directed Coevolution of Two Cellulosic Enzymes in Escherichia coli Based on Their Synergistic Reactions

      • Min Liu, Lidan Ye, Hongwei Yu
      Pages 255-267

    7. Program-Guided Design of High-Throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation

      • Mark Dörr, Uwe T. Bornscheuer
      Pages 269-282
Back to top

Keywords

About this book

This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. 

The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com. 


Editors and Affiliations

  • Department of Biotechnology and Enzyme Catalysis Institute of Biochemistry, Greifswald University, Greifswald, Germany

    Uwe T. Bornscheuer

  • Protein Biochemistry Institute of Biochemistry, Greifswald University, Greifswald, Germany

    Matthias Höhne

Bibliographic Information

  • Book Title: Protein Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Uwe T. Bornscheuer, Matthias Höhne

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7366-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7364-4Published: 30 October 2017

  • Softcover ISBN: 978-1-4939-8463-3Published: 23 May 2018

  • eBook ISBN: 978-1-4939-7366-8Published: 27 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 350

  • Number of Illustrations: 19 b/w illustrations, 63 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation