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Methods in Molecular Biology

Protein Engineering

Methods and Protocols

Editors: Bornscheuer, Uwe T., Höhne, Matthias (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-7366-8
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Hardcover $139.00
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Softcover $139.00
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About this book

This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. 

The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com. 


Table of contents (21 chapters)

Table of contents (21 chapters)
  • Protein Engineering: Past, Present, and Future

    Pages 1-12

    Lutz, Stefan (et al.)

  • Rational and Semirational Protein Design

    Pages 15-23

    Korendovych, Ivan V.

  • Computational Analysis of Protein Tunnels and Channels

    Pages 25-42

    Brezovsky, Jan (et al.)

  • YASARA: A Tool to Obtain Structural Guidance in Biocatalytic Investigations

    Pages 43-67

    Land, Henrik (et al.)

  • A Computational Library Design Protocol for Rapid Improvement of Protein Stability: FRESCO

    Pages 69-85

    Wijma, Hein J. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-7366-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA in USD
Softcover $139.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Protein Engineering
Book Subtitle
Methods and Protocols
Editors
  • Uwe T. Bornscheuer
  • Matthias Höhne
Series Title
Methods in Molecular Biology
Series Volume
1685
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7366-8
DOI
10.1007/978-1-4939-7366-8
Hardcover ISBN
978-1-4939-7364-4
Softcover ISBN
978-1-4939-8463-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 350
Number of Illustrations
19 b/w illustrations, 63 illustrations in colour
Topics