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Methods in Molecular Biology

Flow Cytometry Protocols

Editors: Hawley, Teresa S., Hawley, Robert G. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $119.00
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  • ISBN 978-1-4939-7346-0
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Hardcover $169.99
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  • ISBN 978-1-4939-7344-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA in USD
  • ISBN 978-1-4939-8459-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition  researchers and scientists who are interested in continuing or expanding their knowledge of flow cytometry.

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Table of contents (20 chapters)

Table of contents (20 chapters)
  • Flow Cytometry: The Glass Is Half Full

    Pages 1-10

    Shapiro, Howard M.

  • High-Dimensional Modeling for Cytometry: Building Rock Solid Models Using GemStone™ and Verity Cen-se’™ High-Definition t-SNE Mapping

    Pages 11-36

    Bruce Bagwell, C.

  • Mass Cytometry Assays for Antigen-Specific T Cells Using CyTOF

    Pages 37-47

    Lin, Dongxia (et al.)

  • RNA Flow Cytometry Using the Branched DNA Technique

    Pages 49-77

    Soh, Kah Teong (et al.)

  • Analysis of Individual Extracellular Vesicles by Flow Cytometry

    Pages 79-92

    Nolan, John P. (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7346-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-7344-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA in USD
  • ISBN 978-1-4939-8459-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Flow Cytometry Protocols
Editors
  • Teresa S. Hawley
  • Robert G. Hawley
Series Title
Methods in Molecular Biology
Series Volume
1678
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7346-0
DOI
10.1007/978-1-4939-7346-0
Hardcover ISBN
978-1-4939-7344-6
Softcover ISBN
978-1-4939-8459-6
Series ISSN
1064-3745
Edition Number
4
Number of Pages
XIV, 492
Number of Illustrations
27 b/w illustrations, 80 illustrations in colour
Topics