Bacteriophages
Methods and Protocols, Volume 3
Editors: Clokie, Martha R. J., Kropinski, Andrew, Lavigne, Rob (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater; encapsulation of Listeria phage A511 by alginate; examining genome termini of bacteriophage through high-throughput sequencing; genome sequencing of dsDNA-containing bacteriophages directly from a single plaque; characterizing bacteriophages by biology, taxonomy, and genome analysis; phage genome annotation using the RAST pipeline; and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III is a valuable resource for both established and novice phage scientists.
- Table of contents (22 chapters)
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Basic Phage Mathematics
Pages 3-30
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Analysis of Host-Takeover During SPO1 Infection of Bacillus subtilis
Pages 31-39
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Practical Advice on the One-Step Growth Curve
Pages 41-47
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Iron Chloride Flocculation of Bacteriophages from Seawater
Pages 49-57
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Purification of Bacteriophages Using Anion-Exchange Chromatography
Pages 59-69
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Table of contents (22 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Bacteriophages
- Book Subtitle
- Methods and Protocols, Volume 3
- Editors
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- Martha R. J. Clokie
- Andrew Kropinski
- Rob Lavigne
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1681
- Copyright
- 2018
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-7343-9
- DOI
- 10.1007/978-1-4939-7343-9
- Hardcover ISBN
- 978-1-4939-7341-5
- Softcover ISBN
- 978-1-4939-8458-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 316
- Number of Illustrations
- 25 b/w illustrations, 36 illustrations in colour
- Topics
