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Methods in Molecular Biology

Argonaute Proteins

Methods and Protocols

Editors: Okamura, Katsutomo, Nakanishi, Kotaro (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-7339-2
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Hardcover $169.99
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Softcover $169.99
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About this book

This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Argonaute Proteins: Methods and Protocols is a valuable resource for researchers and scientists looking to expand their knowledge of Argonaute proteins and their functions.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Cloning and Identification of Recombinant Argonaute-Bound Small RNAs Using Next-Generation Sequencing

    Pages 1-28

    Gangras, Pooja (et al.)

  • Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins Using SYBR Green-Based qRT-PCR

    Pages 29-40

    Phan, Hong-Duc (et al.)

  • Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples

    Pages 41-63

    Goh, Eling (et al.)

  • Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant

    Pages 65-73

    Shigematsu, Megumi (et al.)

  • MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans

    Pages 75-86

    Andachi, Yoshiki (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-7339-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Argonaute Proteins
Book Subtitle
Methods and Protocols
Editors
  • Katsutomo Okamura
  • Kotaro Nakanishi
Series Title
Methods in Molecular Biology
Series Volume
1680
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7339-2
DOI
10.1007/978-1-4939-7339-2
Hardcover ISBN
978-1-4939-7338-5
Softcover ISBN
978-1-4939-8457-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 260
Number of Illustrations
11 b/w illustrations, 44 illustrations in colour
Topics