Methods in Molecular Biology

Synthetic Metabolic Pathways

Methods and Protocols

Editors: Jensen, Michael Krogh, Keasling, Jay D (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7295-1
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Hardcover $199.99
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Softcover $139.99
price for USA in USD
  • ISBN 978-1-4939-8445-9
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About this book

This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance. Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Synthetic Metabolic Pathways: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Parts Characterization for Tunable Protein Expression

    Pages 3-14

    Klausen, Michael S. (et al.)

  • Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design

    Pages 15-26

    Cahn, Jackson K. B. (et al.)

  • Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability

    Pages 27-37

    Lauritsen, Ida (et al.)

  • An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization

    Pages 39-61

    Halper, Sean M. (et al.)

  • Computational Approaches on Stoichiometric and Kinetic Modeling for Efficient Strain Design

    Pages 63-82

    Islam, Mohammad Mazharul (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-7295-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-7294-4
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $139.99
price for USA in USD
  • ISBN 978-1-4939-8445-9
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Synthetic Metabolic Pathways
Book Subtitle
Methods and Protocols
Editors
  • Michael Krogh Jensen
  • Jay D Keasling
Series Title
Methods in Molecular Biology
Series Volume
1671
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-4939-7295-1
DOI
10.1007/978-1-4939-7295-1
Hardcover ISBN
978-1-4939-7294-4
Softcover ISBN
978-1-4939-8445-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 354
Number of Illustrations
4 b/w illustrations, 64 illustrations in colour
Topics