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Methods in Molecular Biology

Plant Germline Development

Methods and Protocols

Editors: Schmidt, Anja (Ed.)

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  • Includes cutting-edge methods and protocols for the study of plant germline development
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-7286-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover $179.99
price for USA in USD
Softcover $139.00
price for USA in USD
About this book

This detailed volume explores common and numerous specialized methods to study various aspects of plant germline development and targeted manipulation, including imaging and hybridization techniques to study cell-type specification, cell lineage, signaling and hormones, cell cycle, and the cytoskeleton. In addition, cell-type specific methods for targeted ablation or isolation are provided, protocols to apply “omics” technologies and to perform bioinformatics data analysis, as well as methods relevant for aspects of biotechnology or plant breeding. This includes protocols that are relevant for the targeted manipulation of pathways, for crop plant transformation, or for conditional induction of phenotypes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Plant Germline Development: Methods and Protocols serves as a comprehensive guide not only to studying basic questions related to different aspects of plant reproductive development but also for state of the art methods, in addition to being a source of inspiration for new approaches and research questions in many laboratories.

Table of contents (30 chapters)

Table of contents (30 chapters)
  • An Introduction to Male Germline Development

    Pages 3-15

    Vogler, Hannes (et al.)

  • Apomixis: Engineering the Ability to Harness Hybrid Vigor in Crop Plants

    Pages 17-34

    Conner, Joann A. (et al.)

  • 3D Imaging of Whole-Mount Ovules at Cellular Resolution to Study Female Germline Development in Rice

    Pages 37-45

    Mendocilla-Sato, Ethel (et al.)

  • Live-Cell Imaging of F-Actin Dynamics During Fertilization in Arabidopsis thaliana

    Pages 47-54

    Susaki, Daichi (et al.)

  • Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers

    Pages 55-65

    Chamberlin, Mark A. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-7286-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover $179.99
price for USA in USD
Softcover $139.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Plant Germline Development
Book Subtitle
Methods and Protocols
Editors
  • Anja Schmidt
Series Title
Methods in Molecular Biology
Series Volume
1669
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7286-9
DOI
10.1007/978-1-4939-7286-9
Hardcover ISBN
978-1-4939-7285-2
Softcover ISBN
978-1-4939-8442-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 422
Number of Illustrations
17 b/w illustrations, 86 illustrations in colour
Topics