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Selenoproteins

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Laurent Chavatte

    1. Centre International de Recherche en Infectiologie, CIRI, Lyon, France

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key note and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1661)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Bioinformatic Tools for Selenoprotein Identification and Evolution

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. SECISearch3 and Seblastian: In-Silico Tools to Predict SECIS Elements and Selenoproteins

      • Marco Mariotti
      Pages 3-16

    3. Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins

      • Didac Santesmasses, Marco Mariotti, Roderic Guigó
      Pages 17-28

    4. SelGenAmic: An Algorithm for Selenoprotein Gene Assembly

      • Liang Jiang, Qiong Liu
      Pages 29-39

  3. Selenoprotein Biosynthesis

    1. Front Matter

      Pages 41-41
      Download chapter PDF

    2. Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing

      • Bradley A. Carlson, Byeong Jae Lee, Petra A. Tsuji, Paul R. Copeland, Ulrich Schweizer, Vadim N. Gladyshev et al.
      Pages 43-60

    3. Identification and Characterization of Proteins that Bind to Selenoprotein 3′ UTRs

      • Eric M. Cockman, Donna M. Driscoll
      Pages 61-71

    4. Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis

      • Olga Kossinova, Alexey Malygin, Alain Krol, Galina Karpova
      Pages 73-92

    5. In Vitro Translation Assays for Selenocysteine Insertion

      • Mark H. Pinkerton, Paul R. Copeland
      Pages 93-101

    6. Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profiling

      • Brian K. Dalley, Lisa Baird, Michael T. Howard
      Pages 103-123

    7. Modification of Selenoprotein mRNAs by Cap Tri-methylation

      • Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang
      Pages 125-141

  4. Selenoprotein Detection and Quantification

    1. Front Matter

      Pages 143-143
      Download chapter PDF

    2. Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

      • Véronique Vacchina, Jean Dumont
      Pages 145-152

    3. Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS)

      • Véronique Vacchina, Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski
      Pages 153-162

    4. Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum

      • Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera, José Luis Gómez-Ariza
      Pages 163-175

    5. Radioactive 75Se Labeling and Detection of Selenoproteins

      • Sun Hee Yim, Ryuta Tobe, Anton A. Turanov, Bradley A. Carlson
      Pages 177-192

    6. Nonradioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines

      • Jordan Sonet, Sandra Mounicou, Laurent Chavatte
      Pages 193-203

    7. Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips

      • Jordan Sonet, Sandra Mounicou, Laurent Chavatte
      Pages 205-217

    8. Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues

      • Elisa Castañeda Santa Cruz, J. Susanne Becker, J. Sabine Becker, Alessandra Sussulini
      Pages 219-227
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Keywords

About this book

This volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Centre International de Recherche en Infectiologie, CIRI, Lyon, France

    Laurent Chavatte

Bibliographic Information

  • Book Title: Selenoproteins

  • Book Subtitle: Methods and Protocols

  • Editors: Laurent Chavatte

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7258-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7257-9Published: 16 September 2017

  • Softcover ISBN: 978-1-4939-8433-6Published: 18 May 2018

  • eBook ISBN: 978-1-4939-7258-6Published: 15 September 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 340

  • Number of Illustrations: 28 b/w illustrations, 35 illustrations in colour

  • Topics: Protein Science

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