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Methods in Molecular Biology

c-di-GMP Signaling

Methods and Protocols

Editors: Sauer, Karin (Ed.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7240-1
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-7239-5
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  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
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  • ISBN 978-1-4939-8427-5
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About this book

This volume provides a collection of protocols for the common experimental approaches used in the in the burgeoning field of c-di-GMP-dependent signaling. The chapters, divided into eight major parts, guide readers through methods on synthesis, detection, quantitation, modulation of the levels of c-di-GMP present in cells, procedures to detect and evaluate the interaction of c-di-GMP, and up and coming approaches focusing on the inhibition of c-di-GMP signaling.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, c-di-GMP Signaling: Methods and Protocols aims to inspire researchers to try new approaches.

Table of contents (34 chapters)

Table of contents (34 chapters)
  • Discovery of the Second Messenger Cyclic di-GMP

    Pages 1-8

    Römling, Ute (et al.)

  • Enzymatic Production of c-di-GMP Using a Thermophilic Diguanylate Cyclase

    Pages 11-22

    Venkataramani, Prabhadevi (et al.)

  • Synthesis of [32P]-c-di-GMP for Diguanylate Cyclase and Phosphodiesterase Activity Determinations

    Pages 23-29

    Kazmierczak, Barbara I.

  • High-Performance Liquid Chromatography (HPLC)-Based Detection and Quantitation of Cellular c-di-GMP

    Pages 33-43

    Petrova, Olga E. (et al.)

  • Identification and Quantification of Cyclic Di-Guanosine Monophosphate and Its Linear Metabolites by Reversed-Phase LC-MS/MS

    Pages 45-58

    Bähre, Heike (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7240-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-7239-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8427-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
c-di-GMP Signaling
Book Subtitle
Methods and Protocols
Editors
  • Karin Sauer
Series Title
Methods in Molecular Biology
Series Volume
1657
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7240-1
DOI
10.1007/978-1-4939-7240-1
Hardcover ISBN
978-1-4939-7239-5
Softcover ISBN
978-1-4939-8427-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 486
Number of Illustrations
32 b/w illustrations, 64 illustrations in colour
Topics