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c-di-GMP Signaling

Methods and Protocols

  • Book
  • © 2017

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1657)

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Table of contents (34 protocols)

  1. Synthesis of c-di-GMP

  2. Visualizing c-di-GMP Levels Using Biosensors

  3. Modulation of c-di-GMP Levels and Bacterial Responses

Keywords

About this book

This volume provides a collection of protocols for the common experimental approaches used in the in the burgeoning field of c-di-GMP-dependent signaling. The chapters, divided into eight major parts, guide readers through methods on synthesis, detection, quantitation, modulation of the levels of c-di-GMP present in cells, procedures to detect and evaluate the interaction of c-di-GMP, and up and coming approaches focusing on the inhibition of c-di-GMP signaling.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, c-di-GMP Signaling: Methods and Protocols aims to inspire researchers to try new approaches.

Editors and Affiliations

  • Department of Biological Sciences, Binghamton University Department of Biological Sciences, Binghamton, USA

    Karin Sauer

Bibliographic Information

  • Book Title: c-di-GMP Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Karin Sauer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7240-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7239-5Published: 10 September 2017

  • Softcover ISBN: 978-1-4939-8427-5Published: 11 August 2018

  • eBook ISBN: 978-1-4939-7240-1Published: 09 September 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 486

  • Number of Illustrations: 32 b/w illustrations, 64 illustrations in colour

  • Topics: Cell Biology

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