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Mammalian Synthetic Promoters

  • Book
  • © 2017

Overview

Editors:
  1. David Gould

    1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

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  • Includes cutting-edge methods protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1651)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Initial Considerations Before Designing a Promoter Construct

    • David Gould
    Pages 1-7

  3. Monitoring Promoter Interactions and Activity

    1. Front Matter

      Pages 9-9
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    2. Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay

      • Nasim Yousaf, David Gould
      Pages 11-21

    3. Chromatin Immunoprecipitation and Quantitative Real-Time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites Within a Promoter

      • Jordan E. Read
      Pages 23-32

    4. Secreted Reporters for Monitoring Multiple Promoter Function

      • Ghazal Lashgari, Rami S. Kantar, Bakhos A. Tannous
      Pages 33-47

    5. Bioluminescence Monitoring of Promoter Activity In Vitro and In Vivo

      • Juliette M. K. M. Delhove, Rajvinder Karda, Kate E. Hawkins, Lorna M. FitzPatrick, Simon N. Waddington, Tristan R. McKay
      Pages 49-64

    6. Monitoring Promoter Activity by Flow Cytometry

      • Taher E. I. Taher
      Pages 65-73

  4. Promoter Design and Applications

    1. Front Matter

      Pages 75-75
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    2. Functional Screening of Core Promoter Activity

      • Dan Y. Even, Adi Kedmi, Diana Ideses, Tamar Juven-Gershon
      Pages 77-91

    3. Bioinformatically Informed Design of Synthetic Mammalian Promoters

      • Michael L. Roberts, Polyxeni Katsoupi, Vivian Tseveleki, Era Taoufik
      Pages 93-112

    4. Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication

      • Maria Veronica Lopez, Eduardo G. Cafferata, Diego L. Viale, Osvaldo L. Podhajcer
      Pages 113-130

    5. Constructing Strong Cell Type-Specific Promoters Through Informed Design

      • Adam J. Brown, David C. James
      Pages 131-145

    6. PCR Assembly of Synthetic Promoters

      • Hodan Mohamed, David Gould
      Pages 147-156

  5. Regulated Promoter Approaches

    1. Front Matter

      Pages 157-157
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    2. The Tetracycline Responsive System

      • Nasim Yousaf, David Gould
      Pages 159-172

    3. Light-Responsive Promoters

      • Maximilian Hörner, Konrad Müller, Wilfried Weber
      Pages 173-186

    4. A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation

      • Ryohei Ogawa, Go Kagiya, Akihiko Watanabe, Akihiro Morii, Zheng-Guo Cui, Takashi Kondo
      Pages 187-203

    5. Promoter Activation with Electromagnetism

      • Abraham O. Rodríguez-De la Fuente, J. Antonio Heredia-Rojas, Juan M. Alcocer-González, Laura E. Rodríguez-Flores, Cristina Rodríguez-Padilla, Reyes S. Taméz-Guerra
      Pages 205-212

    6. Application of Synthetic Tumor-Specific Promoters Responsive to the Tumor Microenvironment

      • Eduardo G. Cafferata, Maria Veronica Lopez, Felipe J. Nuñez, Maria A. R. Maenza, Osvaldo L. Podhajcer
      Pages 213-227
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Keywords

About this book

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.

Editors and Affiliations

  • Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

    David Gould

Bibliographic Information

  • Book Title: Mammalian Synthetic Promoters

  • Editors: David Gould

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7223-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7221-0Published: 12 August 2017

  • Softcover ISBN: 978-1-4939-8421-3Published: 12 June 2018

  • eBook ISBN: 978-1-4939-7223-4Published: 11 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 277

  • Number of Illustrations: 18 b/w illustrations, 25 illustrations in colour

  • Topics: Bioinformatics

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