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Methods in Molecular Biology

RNA Detection

Methods and Protocols

Editors: Gaspar, Imre (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD
  • ISBN 978-1-4939-7213-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
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Softcover $199.99
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About this book

This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

Table of contents (32 chapters)

Table of contents (32 chapters)
  • The Secret Life of RNA: Lessons from Emerging Methodologies

    Pages 1-28

    Medioni, Caroline (et al.)

  • Quantification of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)

    Pages 29-48

    Ayadi, Lilia (et al.)

  • Identifying the m6A Methylome by Affinity Purification and Sequencing

    Pages 49-57

    Hsu, Phillip J. (et al.)

  • PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution

    Pages 59-84

    Lu, Zhipeng (et al.)

  • Axon-TRAP-RiboTag: Affinity Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo

    Pages 85-94

    Shigeoka, Toshiaki (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-4939-7213-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $199.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
RNA Detection
Book Subtitle
Methods and Protocols
Editors
  • Imre Gaspar
Series Title
Methods in Molecular Biology
Series Volume
1649
Copyright
2018
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7213-5
DOI
10.1007/978-1-4939-7213-5
Hardcover ISBN
978-1-4939-7212-8
Softcover ISBN
978-1-4939-8418-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 492
Number of Illustrations
16 b/w illustrations, 78 illustrations in colour
Topics