Methods in Molecular Biology

mRNA Processing

Methods and Protocols

Editors: Shi, Yongsheng (Ed.)

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  • Includes cutting-edge methods and protocols for the study of mRNA processing
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-4939-7204-3
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Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-7203-6
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  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems. Given that the vast majority of alternative mRNA processing events has yet to be explored, these techniques promise to breathe new life into the myriad new ideas currently underway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Practical and easy to use, mRNA Processing: Methods and Protocols seeks to aid scientists venturing into what’s certain to be another explosive period of growth in this dynamic field of study.
The chapter 'Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)' is published open access under a CC BY 4.0 license at link.springer.com. 

Table of contents (19 chapters)

  • Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction

    Ho, C. Kiong

    Pages 1-9

  • Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay

    Movassat, Maliheh (et al.)

    Pages 11-26

  • Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS)

    Braun, Joerg E. (et al.)

    Pages 27-37

  • In Vitro Modulation of Endogenous Alternative Splicing Using Splice-Switching Antisense Oligonucleotides

    Park, Jeong Eun (et al.)

    Pages 39-52

  • Purification of mRNA Processing Complexes Using an RNA Affinity Approach

    Wang, Xiuye (et al.)

    Pages 53-63

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7204-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-7203-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
mRNA Processing
Book Subtitle
Methods and Protocols
Editors
  • Yongsheng Shi
Series Title
Methods in Molecular Biology
Series Volume
1648
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7204-3
DOI
10.1007/978-1-4939-7204-3
Hardcover ISBN
978-1-4939-7203-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 258
Number of Illustrations
16 b/w illustrations, 33 illustrations in colour
Topics