- Includes cutting-edge methods and protocols for the study of mRNA processing
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
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This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems. Given that the vast majority of alternative mRNA processing events has yet to be explored, these techniques promise to breathe new life into the myriad new ideas currently underway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy to use, mRNA Processing: Methods and Protocols seeks to aid scientists venturing into what’s certain to be another explosive period of growth in this dynamic field of study.
The chapter 'Robust, Cost-Effective Profiling of RNA Binding Protein Targets with Single-end Enhanced Crosslinking and Immunoprecipitation (seCLIP)' is published open access under a CC BY 4.0 license at link.springer.com.
- Table of contents (19 chapters)
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Detection and Identification of Uncapped RNA by Ligation-Mediated Reverse Transcription Polymerase Chain Reaction
Pages 1-9
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Preparation of Splicing Competent Nuclear Extract from Mammalian Cells and In Vitro Pre-mRNA Splicing Assay
Pages 11-26
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Single-Molecule Analysis of Pre-mRNA Splicing with Colocalization Single-Molecule Spectroscopy (CoSMoS)
Pages 27-37
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In Vitro Modulation of Endogenous Alternative Splicing Using Splice-Switching Antisense Oligonucleotides
Pages 39-52
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Purification of mRNA Processing Complexes Using an RNA Affinity Approach
Pages 53-63
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Table of contents (19 chapters)
- Download Preface 1 PDF (86.6 KB)
- Download Sample pages 1 PDF (289.1 KB)
- Download Table of contents PDF (132.8 KB)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- mRNA Processing
- Book Subtitle
- Methods and Protocols
- Editors
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- Yongsheng Shi
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1648
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-7204-3
- DOI
- 10.1007/978-1-4939-7204-3
- Hardcover ISBN
- 978-1-4939-7203-6
- Softcover ISBN
- 978-1-4939-8415-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 258
- Number of Illustrations
- 16 b/w illustrations, 33 illustrations in colour
- Topics
