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Neuromethods

MicroRNA Technologies

Editors: Jeong Kye, Min (Ed.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains keynotes and implementation advice from the experts

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  • ISBN 978-1-4939-7175-6
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About this book

This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression profiling by PCR array in 2D and 3D neural culture systems; analysis of cortical neuron migration using miRNA silencing; isolating and screening subcellular miRNAs in neurons; and measuring miRNA mediated translational regulation with live cell imaging. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Cutting-edge and practical, MicroRNA Technologies is a valuable resource for students and researchers who are interested about microRNAs in nervous systems.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord

    Pages 1-10

    Reichenstein, Irit (et al.)

  • Protocol for High-Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor Neurons

    Pages 11-19

    Yardeni, Tal (et al.)

  • Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-radioactive Fluorescence Method

    Pages 21-27

    Emde, Anna (et al.)

  • Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Propofol

    Pages 29-42

    Twaroski, Danielle (et al.)

  • MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation

    Pages 43-57

    Stevanato, Lara (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-7175-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-7173-2
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8407-7
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
MicroRNA Technologies
Editors
  • Min Jeong Kye
Series Title
Neuromethods
Series Volume
128
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7175-6
DOI
10.1007/978-1-4939-7175-6
Hardcover ISBN
978-1-4939-7173-2
Softcover ISBN
978-1-4939-8407-7
Series ISSN
0893-2336
Edition Number
1
Number of Pages
XII, 216
Number of Illustrations
10 b/w illustrations, 10 illustrations in colour
Topics

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