Methods in Molecular Biology

Site-Specific Recombinases

Methods and Protocols

Editors: Eroshenko, Nikolai (Ed.)

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  • Includes cutting-edge methods and protocolsProvides step-by-step detail for reproducible resultsContains key notes and implementation advice from the experts

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eBook $139.00
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  • ISBN 978-1-4939-7169-5
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-7167-1
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  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
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  • ISBN 978-1-4939-8405-3
  • Free shipping for individuals worldwide
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About this book

This books aims to provide an up-to-date guide to using recombinases in the laboratory.  Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Generating Genetically Modified Mice: A Decision Guide

    Pages 1-19

    Huijbers, Ivo J.

  • Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes

    Pages 21-35

    Pritchard, Colin E. J. (et al.)

  • Building Cre Knockin Rat Lines Using CRISPR/Cas9

    Pages 37-52

    Ma, Yuanwu (et al.)

  • Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases

    Pages 53-67

    Voziyanova, Eugenia (et al.)

  • Use of the DICE (Dual Integrase Cassette Exchange) System

    Pages 69-85

    Farruggio, Alfonso P. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7169-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-7167-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8405-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Site-Specific Recombinases
Book Subtitle
Methods and Protocols
Editors
  • Nikolai Eroshenko
Series Title
Methods in Molecular Biology
Series Volume
1642
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7169-5
DOI
10.1007/978-1-4939-7169-5
Hardcover ISBN
978-1-4939-7167-1
Softcover ISBN
978-1-4939-8405-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 342
Number of Illustrations
25 b/w illustrations, 25 illustrations in colour
Topics