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Site-Specific Recombinases

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Nikolai Eroshenko

    1. Department of Genetics, Harvard Medical School, Boston, USA

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  • Includes cutting-edge methods and protocols.
  • Provides step-by-step detail for reproducible results.
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1642)

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About this book

This books aims to provide an up-to-date guide to using recombinases in the laboratory.  Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.

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Keywords

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Generating Genetically Modified Mice: A Decision Guide

    • Ivo J. Huijbers
    Pages 1-19

  3. Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes

    • Colin E. J. Pritchard, Lona J. Kroese, Ivo J. Huijbers
    Pages 21-35

  4. Building Cre Knockin Rat Lines Using CRISPR/Cas9

    • Yuanwu Ma, Lianfeng Zhang, Xingxu Huang
    Pages 37-52

  5. Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases

    • Eugenia Voziyanova, Rachelle P. Anderson, Yuri Voziyanov
    Pages 53-67

  6. Use of the DICE (Dual Integrase Cassette Exchange) System

    • Alfonso P. Farruggio, Mital S. Bhakta, Michele P. Calos
    Pages 69-85

  7. Ligand-Controlled Site-Specific Recombination in Zebrafish

    • Avinash Chekuru, Veronika Kuscha, Stefan Hans, Michael Brand
    Pages 87-97

  8. Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre

    • Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, Michael T. Chin
    Pages 99-107

  9. Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain

    • Jonathan C. Y. Tang, Stephanie Rudolph, Constance L. Cepko
    Pages 109-126

  10. Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors

    • Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson et al.
    Pages 127-150

  11. Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X

    • Lilya Kopertekh, Joachim Schiemann
    Pages 151-168

  12. Nanoparticle-Mediated Recombinase Delivery into Maize

    • Susana Martin-Ortigosa, Brian G. Trewyn, Kan Wang
    Pages 169-180

  13. Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines

    • Qi Lu, Zhuo-Hua Pan
    Pages 181-194

  14. FLPing Genes On and Off in Drosophila

    • Bonnie M. Weasner, Jinjin Zhu, Justin P. Kumar
    Pages 195-209

  15. Imaging Neural Architecture in Brainbow Samples

    • Douglas H. Roossien, Dawen Cai
    Pages 211-228

  16. Purification and In Vitro Characterization of Zinc Finger Recombinases

    • Femi J. Olorunniji, Susan J. Rosser, W. Marshall Stark
    Pages 229-245

  17. Preparing Mate-Paired Illumina Libraries Using Cre Recombinase

    • Ze Peng, Nandita Nath, Zhiying Zhao, Jeff L. Froula, Jan-Fang Cheng, Feng Chen
    Pages 247-261

  18. Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination

    • Ze Peng, Jeff L. Froula, Jan-Fang Cheng
    Pages 263-284

  19. Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways

    • Wei Liu, Laura R. Tuck, Jon Marles Wright, Yizhi Cai
    Pages 285-302

  20. Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family

    • Femi J. Olorunniji, Christine Merrick, Susan J. Rosser, Margaret C. M. Smith, W. Marshall Stark, Sean D. Colloms
    Pages 303-323
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Editors and Affiliations

  • Department of Genetics, Harvard Medical School, Boston, USA

    Nikolai Eroshenko

Bibliographic Information

  • Book Title: Site-Specific Recombinases

  • Book Subtitle: Methods and Protocols

  • Editors: Nikolai Eroshenko

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7169-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7167-1Published: 17 August 2017

  • Softcover ISBN: 978-1-4939-8405-3Published: 12 May 2018

  • eBook ISBN: 978-1-4939-7169-5Published: 16 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 342

  • Number of Illustrations: 25 b/w illustrations, 25 illustrations in colour

  • Topics: Human Genetics

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