Methods in Molecular Biology

Plant Argonaute Proteins

Methods and Protocols

Editors: Carbonell, Alberto (Ed.)

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  • Includes cutting-edge methods and protocolsProvides step-by-step essential for reproducible resultsContains key notes and implementation advice from the expert 

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eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7165-7
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-7164-0
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  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
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  • ISBN 978-1-4939-8404-6
  • Free shipping for individuals worldwide
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About this book

This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Plant ARGONAUTEs: Features, Functions, and Unknowns

    Pages 1-21

    Carbonell, Alberto

  • Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants

    Pages 23-37

    Ren, Guodong (et al.)

  • In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts

    Pages 39-53

    Iki, Taichiro (et al.)

  • In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants

    Pages 55-71

    Tomari, Yukihide (et al.)

  • Establishment of an In Vivo ARGONAUTE Reporter System in Plants

    Pages 73-91

    Fátyol, Károly (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7165-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-7164-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-4939-8404-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Argonaute Proteins
Book Subtitle
Methods and Protocols
Editors
  • Alberto Carbonell
Series Title
Methods in Molecular Biology
Series Volume
1640
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7165-7
DOI
10.1007/978-1-4939-7165-7
Hardcover ISBN
978-1-4939-7164-0
Softcover ISBN
978-1-4939-8404-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 297
Number of Illustrations
20 b/w illustrations, 27 illustrations in colour
Topics