Plant Argonaute Proteins
Methods and Protocols
Editors: Carbonell, Alberto (Ed.)
Free Preview- Includes cutting-edge methods and protocolsProvides step-by-step essential for reproducible resultsContains key notes and implementation advice from the expert
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- About this book
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This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.
- Table of contents (20 chapters)
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Plant ARGONAUTEs: Features, Functions, and Unknowns
Pages 1-21
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Analysis of the Uridylation of Both ARGONAUTE-Bound MiRNAs and 5′ Cleavage Products of Their Target RNAs in Plants
Pages 23-37
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In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts
Pages 39-53
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In Vitro Analysis of ARGONAUTE-Mediated Target Cleavage and Translational Repression in Plants
Pages 55-71
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Establishment of an In Vivo ARGONAUTE Reporter System in Plants
Pages 73-91
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Table of contents (20 chapters)
- Download Preface 1 PDF (101.2 KB)
- Download Sample pages 1 PDF (420.5 KB)
- Download Table of contents PDF (1.5 MB)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Plant Argonaute Proteins
- Book Subtitle
- Methods and Protocols
- Editors
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- Alberto Carbonell
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1640
- Copyright
- 2017
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media LLC
- eBook ISBN
- 978-1-4939-7165-7
- DOI
- 10.1007/978-1-4939-7165-7
- Hardcover ISBN
- 978-1-4939-7164-0
- Softcover ISBN
- 978-1-4939-8404-6
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 297
- Number of Illustrations
- 20 b/w illustrations, 27 illustrations in colour
- Topics
