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Methods in Molecular Biology

Circulating Tumor Cells

Methods and Protocols

Editors: M. Magbanua, Mark Jesus, W. Park, John (Eds.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook $149.00
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  • ISBN 978-1-4939-7144-2
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Hardcover $199.99
price for USA in USD
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  • Immediate ebook access, if available*, with your print order
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Softcover $199.99
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  • ISBN 978-1-4939-8397-1
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About this book

This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and authoritative, Circula

ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer of Origin

    Pages 1-19

    Lai, Chian-Hui (et al.)

  • Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability

    Pages 21-32

    Park, Emily S. (et al.)

  • A Novel Microfluidic Device for Isolation of Circulating Tumor Cells from Pancreatic Cancer Blood Samples

    Pages 33-53

    Varillas, Jose I. (et al.)

  • Microfluidic-Based Enrichment and Retrieval of Circulating Tumor Cells for RT-PCR Analysis

    Pages 55-64

    Gogoi, Priya (et al.)

  • Microscale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells

    Pages 65-79

    Hur, Soojung Claire (et al.)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-4939-7144-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-7143-5
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $199.99
price for USA in USD
  • ISBN 978-1-4939-8397-1
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Circulating Tumor Cells
Book Subtitle
Methods and Protocols
Editors
  • Mark Jesus M. Magbanua
  • John W. Park
Series Title
Methods in Molecular Biology
Series Volume
1634
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7144-2
DOI
10.1007/978-1-4939-7144-2
Hardcover ISBN
978-1-4939-7143-5
Softcover ISBN
978-1-4939-8397-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XXV, 306
Number of Illustrations
21 b/w illustrations, 75 illustrations in colour
Topics

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