Methods in Molecular Biology

Fibrosis

Methods and Protocols

Editors: Rittie, Laure (Ed.)

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  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7113-8
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-7112-1
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  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8388-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume describes state-of-the-art protocols that serve as “recipes” for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part II discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Fibrosis: Methods and Protocols is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field.

Table of contents (31 chapters)

Table of contents (31 chapters)
  • Human Fibrotic Diseases: Current Challenges in Fibrosis Research

    Rosenbloom, Joel (et al.)

    Pages 1-23

  • The Bleomycin Model of Pulmonary Fibrosis

    Liu, Tianju (et al.)

    Pages 27-42

  • Intradermal Injections of Bleomycin to Model Skin Fibrosis

    Yamamoto, Toshiyuki

    Pages 43-47

  • Assessing the Effects of Fibrosis on Lung Function by Light Microscopy-Coupled Stereology

    Pilecki, Bartosz (et al.)

    Pages 49-63

  • Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars

    Ding, Jie, M.D., PhD. (et al.)

    Pages 65-80

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7113-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-7112-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $219.99
price for USA in USD
  • ISBN 978-1-4939-8388-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Fibrosis
Book Subtitle
Methods and Protocols
Editors
  • Laure Rittie
Series Title
Methods in Molecular Biology
Series Volume
1627
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7113-8
DOI
10.1007/978-1-4939-7113-8
Hardcover ISBN
978-1-4939-7112-1
Softcover ISBN
978-1-4939-8388-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 530
Number of Illustrations
31 b/w illustrations, 139 illustrations in colour
Topics