Methods in Molecular Biology

The Bacterial Nucleoid

Methods and Protocols

Editors: Espeli, Olivier (Ed.)

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  • Includes cutting-edge methods and protocols
  • ​Includes step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-7098-8
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Hardcover $149.99
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About this book

This volume presents state-of-the-art protocols for key experiments that have revolutionized our understanding of the bacterial nucleoid. This book is divided into five parts: Part I introduces molecular genetic methods to study bacterial nucleoids; Part II highlights the study of bacterial nucleoid with whole genome analysis method; Part III discusses molecular biology methods to study nucleoid structuring factors; Part IV looks at imaging bacterial nucleoid; and Part V explores biophysics of the bacterial nucleoid. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Thorough and cutting-edge, The Bacterial Nucleoid: Methods and Protocols is a valuable resource that provides a wealth of new information about this chromosome.

Table of contents (25 chapters)

Table of contents (25 chapters)
  • Homologous Recombineering to Generate Chromosomal Deletions in Escherichia coli

    Bryant, Jack A. (et al.)

    Pages 3-16

  • Measuring In Vivo Supercoil Dynamics and Transcription Elongation Rates in Bacterial Chromosomes

    Patrick Higgins, N.

    Pages 17-27

  • Revealing Sister Chromatid Interactions with the loxP/Cre Recombination Assay

    Vickridge, Elise (et al.)

    Pages 29-37

  • Transposon Insertion Site Sequencing for Synthetic Lethal Screening

    Yamaichi, Yoshiharu (et al.)

    Pages 39-49

  • WGADseq: Whole Genome Affinity Determination of Protein-DNA Binding Sites

    Poidevin, Mickaël (et al.)

    Pages 53-60

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-7098-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-4939-7097-1
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8384-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Bacterial Nucleoid
Book Subtitle
Methods and Protocols
Editors
  • Olivier Espeli
Series Title
Methods in Molecular Biology
Series Volume
1624
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7098-8
DOI
10.1007/978-1-4939-7098-8
Hardcover ISBN
978-1-4939-7097-1
Softcover ISBN
978-1-4939-8384-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 374
Number of Illustrations
74 b/w illustrations, 82 illustrations in colour
Topics