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  • © 2017

Imaging Mass Spectrometry

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1618)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. Imaging MS of Rodent Ocular Tissues and the Optic Nerve

    • David M. G. Anderson, Wendi Lambert, David J. Calkins, Zsolt Ablonczy, Rosalie K. Crouch, Richard M. Caprioli et al.
    Pages 15-27
  3. MALDI-MSI of Lipids in Human Skin

    • Philippa J. Hart, Malcolm R. Clench
    Pages 29-36
  4. MALDI-MSI Analysis of Cytological Smears: The Study of Thyroid Cancer

    • Niccolò Mosele, Andrew Smith, Manuel Galli, Fabio Pagni, Fulvio Magni
    Pages 37-47
  5. Droplet-Based Liquid Extraction for Spatially-Resolved Microproteomics Analysis of Tissue Sections

    • Maxence Wisztorski, Jusal Quanico, Julien Franck, Benoit Fatou, Michel Salzet, Isabelle Fournier
    Pages 49-63
  6. DESI Mass Spectrometry Imaging (MSI)

    • Emmanuelle Claude, Emrys A. Jones, Steven D. Pringle
    Pages 65-75
  7. MALDI-MS Imaging in the Study of Glomerulonephritis

    • Andrew Smith, Manuel Galli, Vincenzo L’Imperio, Fabio Pagni, Fulvio Magni
    Pages 85-94
  8. Hierarchical Cluster Analysis to Aid Diagnostic Image Data Visualization of MS and Other Medical Imaging Modalities

    • Arul N. Selvan, Laura M. Cole, Lynne Spackman, Sarah Naylor, Chris Wright
    Pages 95-123
  9. Mass Spectrometry Imaging of Drugs of Abuse in Hair

    • Bryn Flinders, Eva Cuypers, Tiffany Porta, Emmanuel Varesio, Gérard Hopfgartner, Ron M. A. Heeren
    Pages 137-147
  10. ToF-SIMS Parallel Imaging MS/MS of Lipid Species in Thin Tissue Sections

    • Anne Lisa Bruinen, Gregory L. Fisher, Ron M. A. Heeren
    Pages 165-173
  11. Back Matter

    Pages 203-204

About this book

This volume addresses the various techniques and novel applications of mass spectrometry imaging (MSI) and its role as a discovery tool in the field of proteomics, lipidomics, and metabolomics. The chapters in this book demonstrate how MSI can be applied to many areas of research such as clinical pathology, translational medicine, toxicology, biomarkers and response studies, and potential incorporation of MSI into forensic workflows. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Innovative and comprehensive, Imaging Mass Spectrometry: Methods and Protocols is a valuable resource for research scientists and clinicians who are interested in further studies of MSI technologies.

Editors and Affiliations

  • The Center for Mass Spectrometry Imaging Biomolecular Science Research Center, Sheffield Hallam University, Sheffield, United Kingdom

    Laura M. Cole

Bibliographic Information

  • Book Title: Imaging Mass Spectrometry

  • Book Subtitle: Methods and Protocols

  • Editors: Laura M. Cole

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7051-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7050-6Published: 19 May 2017

  • Softcover ISBN: 978-1-4939-8378-0Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7051-3Published: 18 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 204

  • Number of Illustrations: 16 b/w illustrations, 68 illustrations in colour

  • Topics: Imaging / Radiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access