Skip to main content
SpringerLink
Log in

Bacterial Protein Secretion Systems

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Laure Journet

    1. Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Eric Cascales

    1. Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1615)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (38 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Identification of Protein Secretion Systems in Bacterial Genomes Using MacSyFinder

    • Sophie S. Abby, Eduardo P. C. Rocha
    Pages 1-21

  3. Protein Sorting Prediction

    • Henrik Nielsen
    Pages 23-57

  4. Cell Fractionation

    • Melissa Petiti, Laetitia Houot, Denis Duché
    Pages 59-64

  5. Defining Lipoprotein Localisation by Fluorescence Microscopy

    • Maria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, Ina Attrée
    Pages 65-74

  6. Identification of Lipoproteins Using Globomycin and Radioactive Palmitate

    • Nienke Buddelmeijer
    Pages 75-80

  7. Defining Membrane Protein Localization by Isopycnic Density Gradients

    • Rhys A. Dunstan, Iain D. Hay, Trevor Lithgow
    Pages 81-86

  8. Cell Surface Exposure

    • Anna Konovalova
    Pages 87-95

  9. Probing Inner Membrane Protein Topology by Proteolysis

    • Maxence S. Vincent, Eric Cascales
    Pages 97-103

  10. Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAM™)

    • Mikhail Bogdanov
    Pages 105-128

  11. Defining Membrane Protein Topology Using pho-lac Reporter Fusions

    • Gouzel Karimova, Daniel Ladant
    Pages 129-142

  12. In Vivo and In Vitro Protein–Peptidoglycan Interactions

    • Gang Li, S. Peter Howard
    Pages 143-149

  13. Measure of Peptidoglycan Hydrolase Activity

    • Yoann G. Santin, Eric Cascales
    Pages 151-158

  14. Protein–Protein Interaction: Bacterial Two-Hybrid

    • Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, Daniel Ladant
    Pages 159-176

  15. Protein–Protein Interactions: Yeast Two-Hybrid System

    • Jer-Sheng Lin, Erh-Min Lai
    Pages 177-187

  16. Protein–Protein Interactions: Cytology Two-Hybrid

    • Krishnamohan Atmakuri
    Pages 189-197

  17. Fusion Reporter Approaches to Monitoring Transmembrane Helix Interactions in Bacterial Membranes

    • Laureen Logger, Abdelrahim Zoued, Eric Cascales
    Pages 199-210

  18. Protein–Protein Interactions: Co-Immunoprecipitation

    • Jer-Sheng Lin, Erh-Min Lai
    Pages 211-219

  19. Protein–Protein Interaction: Tandem Affinity Purification in Bacteria

    • Julie P. M. Viala, Emmanuelle Bouveret
    Pages 221-232

  20. Site-Directed and Time-Resolved Photocrosslinking in Cells Metabolically Labeled with Radioisotopes

    • Raffaele Ieva
    Pages 233-245
Back to top

Keywords

About this book

This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.

Editors and Affiliations

  • Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    Laure Journet, Eric Cascales

Bibliographic Information

  • Book Title: Bacterial Protein Secretion Systems

  • Book Subtitle: Methods and Protocols

  • Editors: Laure Journet, Eric Cascales

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7033-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7031-5Published: 01 July 2017

  • Softcover ISBN: 978-1-4939-8374-2Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7033-9Published: 01 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 528

  • Number of Illustrations: 26 b/w illustrations, 70 illustrations in colour

  • Topics: Microbiology

Publish with us

Policies and ethics

Back to top

Search

Navigation