Methods in Molecular Biology

The Renin-Angiotensin-Aldosterone System

Methods and Protocols

Editors: Thatcher, Sean E. (Ed.)

Free Preview
  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-7030-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
About this book

This volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides and discerning the influence of RAAS components on different mammalian diseases. The chapters cover topics such as the use of fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta, blood pressure monitoring using the radio telemetry method, and the analysis of angiotensin metabolism in the kidney using mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and comprehensive, The Renin-Angiotensin-Aldosterone Systems: Methods and Protocols is a valuable resource that provides scientists and researchers with the best approaches to examine RAAS.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques

    Pages 1-19

    Thatcher, Sean E., Ph.D.

  • A Color Segmentation-Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining

    Pages 21-30

    Wu, Congqing (et al.)

  • Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) Using Immunoblotting Approaches

    Pages 31-46

    Tsiropoulou, Sofia (et al.)

  • Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells

    Pages 47-59

    Jarajapu, Yagna P. R.

  • Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta

    Pages 61-67

    Wang, Yu (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-7030-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
The Renin-Angiotensin-Aldosterone System
Book Subtitle
Methods and Protocols
Editors
  • Sean E. Thatcher
Series Title
Methods in Molecular Biology
Series Volume
1614
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-7030-8
DOI
10.1007/978-1-4939-7030-8
Hardcover ISBN
978-1-4939-7028-5
Softcover ISBN
978-1-4939-8373-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 201
Number of Illustrations
20 b/w illustrations, 38 illustrations in colour
Topics