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Methods in Molecular Biology

Poly(ADP-Ribose) Polymerase

Methods and Protocols

Editors: Tulin, Alexei V. (Ed.)

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  • Includes updated, cutting-edge methods and protocols for the study of PARP research
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook $119.00
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  • ISBN 978-1-4939-6993-7
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Hardcover $159.00
price for USA in USD
  • ISBN 978-1-4939-6992-0
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA in USD
  • ISBN 978-1-4939-8361-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book presents multiple new and classical methods for studying the vital poly-ADP-ribose (pADPr) pathway. Beginning with techniques for the detection and quantification of the product of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr production during the cell cycle, the volume continues with sections on the identification of pADPr protein acceptors, methods focusing on studying molecular mechanisms of PARP functions in eukaryotic cells, particularly those involved in control of DNA repair and oxidative stress, as well as in expression regulation, approaches to the in vitro reconstitution of PARP-1 interaction with chromatin, the development and testing of small molecule PARP inhibitors, and the functions of understudied members of PARP family.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Poly(ADP-Ribose) Polymerase: Methods and Protocols, Second Edition serves as an ideal companion to the first edition for scientists whose investigations involve this important pathway.
The chapter 'Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach' is published open access under a CC BY 4.0 license.

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Quantitation of Poly(ADP-Ribose) by Isotope Dilution Mass Spectrometry

    Pages 3-18

    Zubel, Tabea (et al.)

  • Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies

    Pages 19-26

    Horvath, Eszter M., M.D. Ph.D. (et al.)

  • Detecting and Quantifying pADPr In Vivo

    Pages 27-43

    Lai, Yi-Chen, M.D. (et al.)

  • Compartment-Specific Poly-ADP-Ribose Formation as a Biosensor for Subcellular NAD Pools

    Pages 45-56

    VanLinden, Magali R. (et al.)

  • Cell Cycle Resolved Measurements of Poly(ADP-Ribose) Formation and DNA Damage Signaling by Quantitative Image-Based Cytometry

    Pages 57-68

    Michelena, Jone (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6993-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.00
price for USA in USD
  • ISBN 978-1-4939-6992-0
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.00
price for USA in USD
  • ISBN 978-1-4939-8361-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Poly(ADP-Ribose) Polymerase
Book Subtitle
Methods and Protocols
Editors
  • Alexei V. Tulin
Series Title
Methods in Molecular Biology
Series Volume
1608
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6993-7
DOI
10.1007/978-1-4939-6993-7
Hardcover ISBN
978-1-4939-6992-0
Softcover ISBN
978-1-4939-8361-2
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XV, 528
Number of Illustrations
35 b/w illustrations, 47 illustrations in colour
Topics