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  • © 2017

Reverse Genetics of RNA Viruses

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1602)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Reverse Genetics for Mammalian Orthoreovirus

    • Johnasha D. Stuart, Matthew B. Phillips, Karl W. Boehme
    Pages 1-10
  3. Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus

    • Mingyuan Han, Hanzhong Ke, Yijun Du, Qingzhan Zhang, Dongwan Yoo
    Pages 29-46
  4. Reverse Genetics of Zika Virus

    • Chao Shan, Xuping Xie, Pei-Yong Shi
    Pages 47-58
  5. Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus

    • Erica Bickerton, Sarah M. Keep, Paul Britton
    Pages 83-102
  6. Rescue of Sendai Virus from Cloned cDNA

    • Shringkhala Bajimaya, Tsuyoshi Hayashi, Toru Takimoto
    Pages 103-110
  7. BAC-Based Recovery of Recombinant Respiratory Syncytial Virus (RSV)

    • Christopher C. Stobart, Anne L. Hotard, Jia Meng, Martin L. Moore
    Pages 111-124
  8. Recovery of a Paramyxovirus, the Human Metapneumovirus, from Cloned cDNA

    • B. G. van den Hoogen, R. A. M. Fouchier
    Pages 125-139
  9. Reverse Genetics of Newcastle Disease Virus

    • Stivalis Cardenas-Garcia, Claudio L. Afonso
    Pages 141-158
  10. Reverse Genetics Systems for Filoviruses

    • Thomas Hoenen, Heinz Feldmann
    Pages 159-170
  11. Lassa Virus Reverse Genetics

    • Luis Martínez-Sobrido, Slobodan Paessler, Juan Carlos de la Torre
    Pages 185-204
  12. Reverse Genetics of Influenza B Viruses

    • Aitor Nogales, Daniel R. Perez, Jefferson Santos, Courtney Finch, Luis Martínez-Sobrido
    Pages 205-238
  13. Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA

    • Daniela Toro-Ascuy, Marcelo Cortez-San Martín
    Pages 239-250
  14. Plasmid-Based Reverse Genetics of Influenza A Virus

    • Daniel R. Perez, Matthew Angel, Ana Silvia Gonzalez-Reiche, Jefferson Santos, Adebimpe Obadan, Luis Martinez-Sobrido
    Pages 251-273
  15. Back Matter

    Pages 275-282

About this book

This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Population Health, Poultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, USA

    Daniel R. Perez

Bibliographic Information

  • Book Title: Reverse Genetics of RNA Viruses

  • Book Subtitle: Methods and Protocols

  • Editors: Daniel R. Perez

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6964-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6962-3Published: 16 May 2017

  • Softcover ISBN: 978-1-4939-8353-7Published: 24 June 2018

  • eBook ISBN: 978-1-4939-6964-7Published: 15 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Number of Illustrations: 20 b/w illustrations, 32 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access