Methods in Molecular Biology

Cell Viability Assays

Methods and Protocols

Editors: Gilbert, Daniel F., Friedrich, Oliver (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-6960-9
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Hardcover $169.99
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Softcover $169.99
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About this book

This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cell Viability Assays: Methods and Protocols aims to ensure successful results in the further study of this vital field.

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Table of contents (24 chapters)

Table of contents (24 chapters)
  • Basic Colorimetric Proliferation Assays: MTT, WST, and Resazurin

    Pages 1-17

    Präbst, Konstantin (et al.)

  • Assaying Cellular Viability Using the Neutral Red Uptake Assay

    Pages 19-26

    Ates, Gamze (et al.)

  • Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry

    Pages 27-41

    Chan, Leo Li-Ying (et al.)

  • High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity

    Pages 43-59

    Ivanov, Delyan P. (et al.)

  • A Protocol for In Vitro High-Throughput Chemical Susceptibility Screening in Differentiating NT2 Stem Cells

    Pages 61-70

    Menzner, Ann-Katrin (et al.)

Buy this book

eBook $129.00
price for USA in USD
  • ISBN 978-1-4939-6960-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $169.99
price for USA in USD
Softcover $169.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Cell Viability Assays
Book Subtitle
Methods and Protocols
Editors
  • Daniel F. Gilbert
  • Oliver Friedrich
Series Title
Methods in Molecular Biology
Series Volume
1601
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6960-9
DOI
10.1007/978-1-4939-6960-9
Hardcover ISBN
978-1-4939-6959-3
Softcover ISBN
978-1-4939-8352-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 299
Number of Illustrations
20 b/w illustrations, 50 illustrations in colour
Topics