Methods in Molecular Biology

Synthetic Protein Switches

Methods and Protocols

Editors: Stein, Viktor (Ed.)

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  • Includes cutting-edge methods and protocols for the study of synthetic protein switches in biotechnology
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook $109.00
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  • ISBN 978-1-4939-6940-1
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Hardcover $149.99
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  • ISBN 978-1-4939-6938-8
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  • Usually dispatched within 3 to 5 business days.
Softcover $149.99
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  • ISBN 978-1-4939-8345-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, β-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Synthetic Protein Switches: Theoretical and Experimental Considerations

    Stein, Viktor

    Pages 3-25

  • Construction of Allosteric Protein Switches by Alternate Frame Folding and Intermolecular Fragment Exchange

    Ha, Jeung-Hoi (et al.)

    Pages 27-41

  • Construction of Protein Switches by Domain Insertion and Directed Evolution

    Ribeiro, Lucas F. (et al.)

    Pages 43-55

  • Catalytic Amyloid Fibrils That Bind Copper to Activate Oxygen

    Sternisha, Alex (et al.)

    Pages 59-68

  • Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors

    Clifton, Ben E. (et al.)

    Pages 71-87

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6940-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-1-4939-6938-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4939-8345-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Synthetic Protein Switches
Book Subtitle
Methods and Protocols
Editors
  • Viktor Stein
Series Title
Methods in Molecular Biology
Series Volume
1596
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6940-1
DOI
10.1007/978-1-4939-6940-1
Hardcover ISBN
978-1-4939-6938-8
Softcover ISBN
978-1-4939-8345-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 341
Number of Illustrations
28 b/w illustrations, 31 illustrations in colour
Topics