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Methods in Molecular Biology

Protein-Carbohydrate Interactions

Methods and Protocols

Editors: Abbott, D. Wade, Lammerts van Bueren, Alicia (Eds.)

  • Includes cutting-edge methods and protocols
    Provides step-by-step detail essential for reproducible results
    Contains keynotes and implementation advice from the experts

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6899-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6898-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume is a wide-ranging tool for studying protein-carbohydrate interactions that extend from traditional biochemical methods to state-of-the-art techniques. This book focuses on four different research themes: Part I describes methods for screening and quantifying CAZyme activity; Part II contains methods for investigating the interactions between proteins and carbohydrate ligands; Part III discusses methods for the visualization of carbohydrates and protein-carbohydrate complexes; and Part IV focuses on structural and “omic” approaches for studying systems of CAZymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein-Carbohydrate Interactions: Methods and Protocols is a valuable resource to the glycomics research community. In this continuously advancing field, the methods in this book highlight the biology of glycomics, thus driving biotechnological innovation and solutions for human health and sustainable resources within the emerging green community.

Table of contents (23 chapters)

  • A Low-Volume, Parallel Copper-Bicinchoninic Acid (BCA) Assay for Glycoside Hydrolases

    Arnal, Gregory (et al.)

    Pages 3-14

  • Quantitative Kinetic Characterization of Glycoside Hydrolases Using High-Performance Anion-Exchange Chromatography (HPAEC)

    McGregor, Nicholas (et al.)

    Pages 15-25

  • Measuring Enzyme Kinetics of Glycoside Hydrolases Using the 3,5-Dinitrosalicylic Acid Assay

    McKee, Lauren S.

    Pages 27-36

  • An Improved Kinetic Assay for the Characterization of Metal-Dependent Pectate Lyases

    Jones, Darryl R. (et al.)

    Pages 37-44

  • Colorimetric Detection of Acetyl Xylan Esterase Activities

    Mai-Gisondi, Galina (et al.)

    Pages 45-57

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6899-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-6898-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein-Carbohydrate Interactions
Book Subtitle
Methods and Protocols
Editors
  • D. Wade Abbott
  • Alicia Lammerts van Bueren
Series Title
Methods in Molecular Biology
Series Volume
1588
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6899-2
DOI
10.1007/978-1-4939-6899-2
Hardcover ISBN
978-1-4939-6898-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 311
Number of Illustrations and Tables
20 b/w illustrations, 42 illustrations in colour
Topics