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Methods in Molecular Biology

Heterologous Gene Expression in E.coli

Methods and Protocols

Editors: Burgess-Brown, Nicola A. (Ed.)

  • Includes cutting-edge methods and protocols for the study of E. coli gene expression
    Provides step-by-step detail essential for reproducible results
    Contains key notes and implementation advice from the experts

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eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6887-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-6885-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8328-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput  cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.

Table of contents (27 chapters)

  • Recombinant Protein Expression in E. coli : A Historical Perspective

    Gileadi, Opher

    Pages 3-10

  • N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools

    Cooper, Christopher D. O. (et al.)

    Pages 11-31

  • Harnessing the Profinity eXact™ System for Expression and Purification of Heterologous Proteins in E. coli

    Peleg, Yoav (et al.)

    Pages 33-43

  • ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences

    Mas, Philippe J. (et al.)

    Pages 45-63

  • Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters

    Taylor, Troy (et al.)

    Pages 65-82

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-6887-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-6885-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4939-8328-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Heterologous Gene Expression in E.coli
Book Subtitle
Methods and Protocols
Editors
  • Nicola A. Burgess-Brown
Series Title
Methods in Molecular Biology
Series Volume
1586
Copyright
2017
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-4939-6887-9
DOI
10.1007/978-1-4939-6887-9
Hardcover ISBN
978-1-4939-6885-5
Softcover ISBN
978-1-4939-8328-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 429
Number of Illustrations and Tables
37 b/w illustrations, 55 illustrations in colour
Topics